ArticleNucleic acids research2005
DNA analysis with multiplex microarray-enhanced PCR.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 64 citations in OpenAlex.
- Development of Multiplex RT-PCR with Immobilized Primers for Identification of Infectious Human Pneumonia Pathogens.Molecular biology · 2021Article
- Biophysics and the Genomic Sciences.Biophysical journal · 2019Review
- Genotyping of KRAS Mutational Status by the In-Check Lab-on-Chip Platform.Sensors (Basel, Switzerland) · 2018Article
- Profiling in situ microbial community structure with an amplification microarray.Applied and environmental microbiology · 2013Article
- Integrated Amplification Microarrays for Infectious Disease Diagnostics.Microarrays (Basel, Switzerland) · 2012Review
- Nonvolatile copolymer compositions for fabricating gel element microarrays.Analytical biochemistry · 2012Article
- Article
- Mechanical disruption of lysis-resistant bacterial cells by use of a miniature, low-power, disposable device.Journal of clinical microbiology · 2011Article
- Fabrication of DNA polymer brush arrays by destructive micropatterning and rolling-circle amplification.Macromolecular bioscience · 2011Article
- Dual primer emulsion PCR for next- generation DNA sequencing.BioTechniques · 2010Article
- New Generations: Sequencing Machines and Their Computational Challenges.Journal of computer science and technology · 2010Article
- The role of DNA diffusion in solid phase polymerase chain reaction with gel-immobilized primers in planar and capillary microarray format.Biomicrofluidics · 2009Article
- Large scale multiplex PCR improves pathogen detection by DNA microarrays.BMC microbiology · 2009Article
- In situ oligonucleotide synthesis on carbon materials: stable substrates for microarray fabrication.Nucleic acids research · 2008Article
- Multiple primer extension by DNA polymerase on a novel plastic DNA array coated with a biocompatible polymer.Nucleic acids research · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a highly sensitive method for DNA analysis on 3D gel element microarrays, a technique we call multiplex microarray-enhanced PCR (MME-PCR). Two amplification strategies are carried out simultaneously in the reaction chamber: on or within gel elements, and in bulk solution over the gel element array. MME-PCR is initiated by multiple complex primers containing gene-specific, forward and reverse, sequences appended to the 3' end of a universal amplification primer. The complex primer pair is covalently tethered through its 5' end to the polyacryl- amide backbone. In the bulk solution above the gel element array, a single pair of unattached universal primers simultaneously directs pseudo-monoplex PCR of all targets according to normal solution-phase PCR. The presence of a single universal PCR primer pair in solution accelerates amplification within gel elements and eliminates the problem of primer interference that is common to conventional multiplex PCR. We show 10(6)-fold amplification of targeted DNA after 50 cycles with average amplification efficiency 1.34 per cycle, and demonstrate specific on-chip amplification of six genes in Bacillus subtilis. All six genes were detected at 4.5 pg of bacterial genomic DNA (equivalent to 10(3) genomes) in 60 independent amplification reactions performed simultaneously in single reaction chamber.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.