ArticleRNA (New York, N.Y.)2005
Conserved spacing between the box C/D and C'/D' RNPs of the archaeal box C/D sRNP complex is required for efficient 2'-O-methylation of target RNAs.
Article in RNA (New York, N.Y.), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 43 citations in OpenAlex.
- The roles of NOP56 in cancer and SCA36.Pathology oncology research : POR · 2023Review
- Eukaryotic Box C/D methylation machinery has two non-symmetric protein assembly sites.Scientific reports · 2021Article
- Emerging Data on the Diversity of Molecular Mechanisms Involving C/D snoRNAs.Non-coding RNA · 2021Review
- High-resolution structure of eukaryotic Fibrillarin interacting with Nop56 amino-terminal domain.RNA (New York, N.Y.) · 2021Article
- Conservation of Archaeal C/D Box sRNA-Guided RNA Modifications.Frontiers in microbiology · 2021Review
- Functional organization of box C/D RNA-guided RNA methyltransferase.Nucleic acids research · 2020Article
- Functional Interplay between Small Non-Coding RNAs and RNA Modification in the Brain.Non-coding RNA · 2018Review
- Phylogenetic distribution of plant snoRNA families.BMC genomics · 2016Article
- Box C/D guide RNAs recognize a maximum of 10 nt of substrates.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Ribonucleoproteins in archaeal pre-rRNA processing and modification.Archaea (Vancouver, B.C.) · 2013Review
- The box C/D sRNP dimeric architecture is conserved across domain Archaea.RNA (New York, N.Y.) · 2012Article
- Programmable sequence-specific click-labeling of RNA using archaeal box C/D RNP methyltransferases.Nucleic acids research · 2012Article
- Structurally conserved Nop56/58 N-terminal domain facilitates archaeal box C/D ribonucleoprotein-guided methyltransferase activity.The Journal of biological chemistry · 2012Article
- Structures of ribonucleoprotein particle modification enzymes.Quarterly reviews of biophysics · 2011Review
- Structural basis for site-specific ribose methylation by box C/D RNA protein complexes.Nature · 2011Article
- The spatial-functional coupling of box C/D and C'/D' RNPs is an evolutionarily conserved feature of the eukaryotic box C/D snoRNP nucleotide modification complex.Molecular and cellular biology · 2011Article
- Dissecting the role of conserved box C/D sRNA sequences in di-sRNP assembly and function.Nucleic acids research · 2010Article
- Ribonucleoprotein multimers and their functions.Critical reviews in biochemistry and molecular biology · 2010Review
- Structural basis for substrate placement by an archaeal box C/D ribonucleoprotein particle.Molecular cell · 2010Article
- Signature amino acids enable the archaeal L7Ae box C/D RNP core protein to recognize and bind the K-loop RNA motif.RNA (New York, N.Y.) · 2010Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA-guided nucleotide modification complexes direct the post-transcriptional nucleotide modification of both archaeal and eukaryotic RNAs. We have previously demonstrated that efficient 2'-O-methylation activity guided by an in vitro reconstituted archaeal box C/D sRNP requires juxtaposed box C/D and C'/D' RNP complexes. In these experiments, we investigate the importance of spatially positioning the box C/D and C'/D' RNPs within the sRNP complex for nucleotide modification. Initial sequence analysis of 245 archaeal box C/D sRNAs from both Eukyarchaeota and Crenarchaeota kingdoms revealed highly conserved spacing between the box C/D and C'/D' RNA motifs. Distances between boxes C to D' and C' to D (D' and D spacers, respectively) exhibit highly constrained lengths of 12 nucleotides (nt). Methanocaldococcus jannaschii sR8 sRNA, a model box C/D sRNA with D and D' spacers of 12 nt, was mutated to alter the distance between the two RNA motifs. sRNAs with longer or shorter spacer regions could still form sRNPs by associating with box C/D core proteins, L7, Nop56/58, and fibrillarin, comparable to wild-type sR8. However, these reconstituted box C/D sRNP complexes were severely deficient in methylation activity. Alteration of the D and D' spacer lengths disrupted the guided methylation activity of both the box C/D and C'/D' RNP complexes. When only one spacer region was altered, methylation activity of the corresponding RNP was lost. Collectively, these results demonstrate the importance of box C/D and C'/D' RNP positioning for preservation of critical inter-RNP interactions required for efficient box C/D sRNP-guided nucleotide methylation.
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