ArticleThe Journal of physiology2005
Simultaneous changes of cell volume and cytosolic calcium concentration in macula densa cells caused by alterations of luminal NaCl concentration.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 33 citations in OpenAlex.
- Aging Impairs Renal Autoregulation in Mice.Hypertension (Dallas, Tex. : 1979) · 2020Article
- Role of the Primary Cilia on the Macula Densa and Thick Ascending Limbs in Regulation of Sodium Excretion and Hemodynamics.Hypertension (Dallas, Tex. : 1979) · 2017Article
- Kidney tubules: intertubular, vascular, and glomerular cross-talk.Current opinion in nephrology and hypertension · 2016Review
- Shear stress blunts tubuloglomerular feedback partially mediated by primary cilia and nitric oxide at the macula densa.American journal of physiology. Regulatory, integrative and comparative physiology · 2015Article
- Oxidative status in the macula densa modulates tubuloglomerular feedback responsiveness in angiotensin II-induced hypertension.Acta physiologica (Oxford, England) · 2015Article
- Renal intercalated cells are rather energized by a proton than a sodium pump.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Analysis of spontaneous and nerve-evoked calcium transients in intact extraocular muscles in vitro.Experimental eye research · 2012Article
- Aldosterone stimulates superoxide production in macula densa cells.American journal of physiology. Renal physiology · 2011Article
- Multiphoton imaging of renal regulatory mechanisms.Physiology (Bethesda, Md.) · 2009Review
- Increased renal renin content in mice lacking the Na+/H+ exchanger NHE2.American journal of physiology. Renal physiology · 2008Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
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Abstract
Cell volume and cytosolic Ca(2+) concentration ([Ca(2+)](i)) were measured in rabbit macula densa (MD) cells loaded with calcein and Fura Red using confocal microscopy. [Ca(2+)](i) was also analysed with Indo-1 and fura-2. We used isolated microperfused thick ascending limbs with attached glomerulus. The results showed that when the luminal NaCl concentration ('NaCl') was decreased from 35 to 10 mM, the cell volume decreased by 10.4%, and [Ca(2+)](i) increased by 9.5%. This increase was inhibited in Ca(2+)-free solution. When luminal [NaCl] was changed from 35 to 135 mM, the cell volume increased by 15.1%, and [Ca(2+)](i) did not change. The cell volume alterations were not different in Ca(2+)-free solutions. Using Indo-1, basal [Ca(2+)](i) in MD cells was 107.8 nM. When luminal [NaCl] was changed from 135 to 10 mm, [Ca(2)](i) increased by 23.5 nM. Using fura-2, the basal [Ca(2+)](i) in MD cells was 115.3 nM, and when luminal [NaCl] was changed from 135 or 35 to 10 mM, [Ca(2+)](i) change was 30.1 or 10.6 nM, respectively. An increase in [NaCl] caused no change in [Ca(2+)](i). In Ca(2+)-free solution, no change in [Ca(2+)](i) occurred. A stepwise decrease in luminal [NaCl] resulted in a sigmoid increase in [Ca(2+)](i) in MD cells. The steepest part of the curve was between 70 and 10 mM. In conclusion, we found that MD cells have cell volume regulation, and that [Ca(2+)](i) elevation caused by decreased luminal [NaCl] is independent of the cell volume.
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