Trial reportThe Journal of physiology2005
Mechanisms of acetylcholine-mediated vasodilatation in young and aged human skin.
Trial report in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06282679 (Clinical Observation of Intradermal Injections With Botulinum Toxin A in the Treatment of Rosacea Erythema Telangiectasia), which is not on this map. Cited by 108 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Clinical Observation of Intradermal Injections With Botulinum Toxin A in the Treatment of Rosacea Erythema Telangiectasia
Who cites it
108 citing papers in PubMed, 219 citations in OpenAlex.
- Salsalate negatively impacts microvascular function in women with endometriosis.American journal of physiology. Heart and circulatory physiology · 2025Trial
- Controlled Feeding of an 8-d, High-Dairy Cheese Diet Prevents Sodium-Induced Endothelial Dysfunction in the Cutaneous Microcirculation of Healthy, Older Adults through Reductions in Superoxide.The Journal of nutrition · 2020Trial
- Effects of supervised exercise training on lower-limb cutaneous microvascular reactivity in adults with venous ulcers.European journal of applied physiology · 2018Trial
- Effects of vehicle microdialysis solutions on cutaneous vascular responses to local heating.Journal of applied physiology (Bethesda, Md. : 1985) · 2017Trial
- Mechanisms and time course of menthol-induced cutaneous vasodilation.Microvascular research · 2017Trial
- Impaired acetylcholine-induced cutaneous vasodilation in young smokers: roles of nitric oxide and prostanoids.American journal of physiology. Heart and circulatory physiology · 2013Trial
- Acute localized administration of tetrahydrobiopterin and chronic systemic atorvastatin treatment restore cutaneous microvascular function in hypercholesterolaemic humans.The Journal of physiology · 2011Trial
- Oral atorvastatin therapy increases nitric oxide-dependent cutaneous vasodilation in humans by decreasing ascorbate-sensitive oxidants.American journal of physiology. Regulatory, integrative and comparative physiology · 2011Trial
- Transient receptor potential vanilloid type-1 (TRPV-1) channels contribute to cutaneous thermal hyperaemia in humans.The Journal of physiology · 2010Trial
- Ketorolac alters blood flow during normothermia but not during hyperthermia in middle-aged human skin.Journal of applied physiology (Bethesda, Md. : 1985) · 2009Trial
- Endothelial nitric oxide synthase control mechanisms in the cutaneous vasculature of humans in vivo.American journal of physiology. Heart and circulatory physiology · 2008Trial
- Cutaneous blood flow and sweat rate responses to exogenous administration of acetylcholine and methacholine.Journal of applied physiology (Bethesda, Md. : 1985) · 2007Trial
- Noninvasive measure of microvascular nitric oxide function in humans using very low-frequency cutaneous laser Doppler flow spectra.Microcirculation (New York, N.Y. : 1994)Trial
- Methods for analyzing acetylcholine-induced vasodilation in human skin.Journal of applied physiology (Bethesda, Md. : 1985) · 2026Article
- Cardiovascular challenges of aging in a hotter environment: a narrative review.Journal of applied physiology (Bethesda, Md. : 1985) · 2025Review
- Intermittent hand and forearm immersion in 20°C water attenuates thermal, cardiovascular, and perceptual strain in older adults during heat stress.Experimental physiology · 2025Article
- Oral contraceptive pill phase alters mechanisms contributing to cutaneous microvascular function in response to local heating.American journal of physiology. Regulatory, integrative and comparative physiology · 2025Article
- Impaired microvascular function in patients with sickle cell anemia and leg ulcers improved with healing.British journal of haematology · 2024Article
- Reduced systemic microvascular function in patients with resistant hypertension and microalbuminuria: an observational study.Journal of human hypertension · 2024Observational
- The Effect of Overweight/Obesity on Cutaneous Microvascular Reactivity as Measured by Laser-Doppler Fluxmetry: A Systematic Review.Biomedicines · 2024Review
48 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Thermoregulatory cutaneous vasodilatation (VD) is attenuated in aged skin. While acetylcholine (ACh) plays a role in thermally mediated VD, the precise mechanisms through which ACh-mediated VD acts and whether those downstream mechanisms change with ageing are unclear. We tested the hypotheses that both nitric oxide (NO)- and prostanoid-mediated pathways contribute to exogenous ACh-mediated VD, and that both are attenuated with advanced age. Twelve young (Y: 23 +/- 1 years) and 10 older (O: 69 +/- 1 years) subjects underwent infusions of 137.5 mum ACh at four intradermal microdialysis sites: control (C, Ringer solution), NO synthase inhibited (NOS-I, 10 mm l-NAME), cyclooxygenase inhibited (COX-I, 10 mm ketorolac) and NOS-I + COX-I. Red blood cell flux was monitored using laser-Doppler flowmetry, and cutaneous vascular conductance (CVC) was calculated (laser-Doppler flux/mean arterial pressure) and normalized to maximal CVC (%CVC(max)) (28 mm sodium nitroprusside + local heating to 43 degrees C). Baseline %CVC(max) was increased in the O at COX-I sites (COX-I 16 +/- 1, NOS-I + COX-I 16 +/- 2 versus C 10 +/- 1%CVC(max); P < 0.001) but not in the young, suggesting an age-related shift toward COX vasoconstrictors contributing to basal cutaneous vasomotor tone. There was no difference in peak %CVC(max) during ACh infusion between age groups, and the response was unchanged by NOS-I (O: NOS-I 35 +/- 5 versus C 38 +/- 5%CVC(max); P = 0.84) (Y: NOS-I 41 +/- 4 versus C 39 +/- 4%CVC(max); P = 0.67). COX-I and NOS-I + COX-I attenuated the peak CVC response to ACh in both groups (COX-I O: 29 +/- 3, Y: 22 +/- 2%CVC(max) versus C; P < 0.001 both groups; NOS-I + COX-I O: 32 +/- 3 versus Y: 29 +/- 2%CVC(max); versus C; P < 0.001 both groups). ACh mediates cutaneous VD through prostanoid and non-NO-, non-prostanoid-dependent pathways. Further, older subjects have a diminished prostanoid contribution to ACh-mediated VD.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.