ArticlePlant cell reports2005
Genetic transformation of Tylophora indica with Agrobacterium rhizogenes A4: growth and tylophorine productivity in different transformed root clones.
Article in Plant cell reports, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 92 citations in OpenAlex.
- Enhancement of Rosmarinic Acid Production in Hairy Root Cultures ofInternational journal of molecular sciences · 2025Article
- Piriformospora indica culture filtrate and cell extract induce chicoric acid production in Echinacea purpurea hairy roots.PloS one · 2025Article
- Benzoindolizidine Alkaloids Tylophorine and Lycorine and Their Analogues with Antiviral, Anti-Inflammatory, and Anticancer Properties: Promises and Challenges.Biomedicines · 2023Review
- Natural Products in Renal-Associated Drug Discovery.Antioxidants (Basel, Switzerland) · 2023Review
- Phenanthroindolizidine Alkaloids Secondary Metabolites Diversity in Medicinally Viable Plants of the GenusPlants (Basel, Switzerland) · 2023Review
- Influence of different3 Biotech · 2018Article
- Neoteric trends in tissue culture-mediated biotechnology of Indian ipecac [Tylophora indica (Burm. f.) Merrill].3 Biotech · 2017Review
- Optimizing culture conditions for establishment of hairy root culture of Semecarpus anacardium L.3 Biotech · 2017Article
- Micropropagation and genetic transformation of Tylophora indica (Burm. f.) Merr.: a review.Plant cell reports · 2016Review
- Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production.Plant cell reports · 2015Article
- Use of the cryptogein gene to stimulate the accumulation of Bacopa saponins in transgenic Bacopa monnieri plants.Plant cell reports · 2012Article
- Genetic transformation of Bacopa monnieri by wild type strains of Agrobacterium rhizogenes stimulates production of bacopa saponins in transformed calli and plants.Plant cell reports · 2011Article
- Transgenic mimicry of pathogen attack stimulates growth and secondary metabolite accumulation.Transgenic research · 2009Article
- Agrobacterium rhizogenes-transformed roots of coffee (Coffea arabica): conditions for long-term proliferation, and morphological and molecular characterization.Annals of botany · 2008Article
- The genetic manipulation of medicinal and aromatic plants.Plant cell reports · 2007Review
- Changes in morphological phenotypes and withanolide composition of Ri-transformed roots of Withania somnifera.Plant cell reports · 2007Article
- Spontaneous plant regeneration in transformed roots and calli from Tylophora indica: Changes in morphological phenotype and tylophorine accumulation associated with transformation by Agrobacterium rhizogenes.Plant cell reports · 2006Article
- Efficient production of Agrobacterium rhizogenes-transformed roots and composite plants for studying gene expression in coffee roots.Plant cell reports · 2006Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed an efficient transformation system for Tylophora indica, an important medicinal plant in India, using Agrobacterium rhizogenes strains LBA9402 and A4 to infect excised leaf and stem explants and intact shoots at different sites. The induction of callus and transformed roots was dependent on the bacterial strain, explant type and inoculation site used. Transformed roots were induced only in explants infected with A. rhizogenes strain A4, while an optimal transformation frequency of up to 60% was obtained with intact shoots inoculated at the nodes. The presence of the left-hand transferred DNA (T(L)-DNA) in the genome of T. indica roots induced by A. rhizogenes was confirmed by PCR amplification of the rooting locus genes of A. rhizogenes. Root growth and the production of tylophorine, the major alkaloid of the plant, varied substantially among the nine root clones studied. Both parameters increased over time in liquid cultures, with maximum biomass and tylophorine accumulation occurring within 4-6 weeks of growth in fresh medium. Interestingly, in liquid culture, the culture medium also accumulated tylophorine up to concentrations of 9.78+/-0.21 mg l(-1).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.