ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2005
TM2, a novel strong matrix attachment region isolated from tobacco, increases transgene expression in transgenic rice calli and plants.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 38 citations in OpenAlex.
- Comparing four heat-inducible promoters in stably transformed sugarcane regarding spatial and temporal control of transgene expression reveals candidates to drive stem-preferred transgene expression.Frontiers in plant science · 2025Article
- Lettuce-based production of an oral vaccine against porcine edema disease for the seed lot system.Plant biotechnology (Tokyo, Japan) · 2021Article
- The M4 insulator, the TM2 matrix attachment region, and the double copy of the heavy chain gene contribute to the enhanced accumulation of the PHB-01 antibody in tobacco plants.Transgenic research · 2020Article
- Extended Set of GoldenBraid Compatible Vectors for Fast Assembly of Multigenic Constructs and Their Use to Create Geminiviral Expression Vectors.Frontiers in plant science · 2020Article
- Review
- BdCESA7, BdCESA8, and BdPMT Utility Promoter Constructs for Targeted Expression to Secondary Cell-Wall-Forming Cells of Grasses.Frontiers in plant science · 2016Article
- 5' and 3' Untranslated Regions Strongly Enhance Performance of Geminiviral Replicons in Nicotiana benthamiana Leaves.Frontiers in plant science · 2016Article
- Co-expression of the Arabidopsis SOS genes enhances salt tolerance in transgenic tall fescue (Festuca arundinacea Schreb.).Protoplasma · 2014Article
- Creation and validation of a widely applicable multiple gene transfer vector system for stable transformation in plant.Plant molecular biology · 2013Article
- TM6, a novel nuclear matrix attachment region, enhances its flanking gene expression through influencing their chromatin structure.Molecules and cells · 2013Article
- Minimizing the unpredictability of transgene expression in plants: the role of genetic insulators.Plant cell reports · 2012Review
- Functional analysis of BnMAR element in transgenic tobacco plants.Molecular biology reports · 2011Article
- MISSA is a highly efficient in vivo DNA assembly method for plant multiple-gene transformation.Plant physiology · 2010Article
- Functional characterization of a tobacco matrix attachment region-mediated enhancement of transgene expression.Transgenic research · 2009Article
- Design of gene constructs for transgenic maize.Methods in molecular biology (Clifton, N.J.) · 2009Review
- The influence of matrix attachment regions on transgene expression in Arabidopsis thaliana wild type and gene silencing mutants.Plant molecular biology · 2007Article
- A Gateway-based platform for multigene plant transformation.Plant molecular biology · 2006Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear matrix attachment regions (MARs) are thought to influence the expression of the flanking genes. TM2, a new DNA fragment isolated from tobacco, can bind with the rice nuclear matrix in vitro. In this study, we investigated the effect of TM2 on transgene expression under the control of three different promoters in stably transformed rice calli and plants. The presence of TM2 flanking the transgene increased the expression of constructs based on the constitutive CaMV 35S and maize ubiquitin gene promoters in both resistant calli and transformed plants. The GUS expression directed by the photosynthetic-tissue-specific PNZIP promoter was also increased in photosynthetic tissues of transformants. However, TM2 did not change the gene expression pattern controlled by the PNZIP promoter. The effect of TM2 in transgenic plants was stronger than that in transgenic calli based on all three promoters. Our results indicate that TM2, as a novel strong MAR, can be used to increase the transgene expression levels in the whole plant or in particular tissues of monocotyledons.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.