ArticleJournal of bacteriology2005
NopB, a type III secreted protein of Rhizobium sp. strain NGR234, is associated with pilus-like surface appendages.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 56 citations in OpenAlex.
- Rhizobial effector NopM mediates the ubiquitination of the Nod factor receptor NFR5 and promotes rhizobial symbiosis in Lotusjaponicus.Plant communications · 2026Article
- The Rhizobial Type 3 Secretion System: The Dr. Jekyll and Mr. Hyde in the Rhizobium-Legume Symbiosis.International journal of molecular sciences · 2022Review
- The Type III Effectome of the SymbioticBiomolecules · 2021Article
- Tailoring plant-associated microbial inoculants in agriculture: a roadmap for successful application.Journal of experimental botany · 2020Review
- QTL Mapping and Data Mining to Identify Genes Associated With theFrontiers in plant science · 2020Article
- The Sinorhizobium (Ensifer) fredii HH103 Nodulation Outer Protein NopI Is a Determinant for Efficient Nodulation of Soybean and Cowpea Plants.Applied and environmental microbiology · 2017Article
- A transcriptomic analysis of the effect of genistein on Sinorhizobium fredii HH103 reveals novel rhizobial genes putatively involved in symbiosis.Scientific reports · 2016Article
- Bacterial Molecular Signals in the Sinorhizobium fredii-Soybean Symbiosis.International journal of molecular sciences · 2016Article
- Secretion systems and signal exchange between nitrogen-fixing rhizobia and legumes.Frontiers in plant science · 2015Review
- NopC Is a Rhizobium-Specific Type 3 Secretion System Effector Secreted by Sinorhizobium (Ensifer) fredii HH103.PloS one · 2015Article
- Commonalities and differences of T3SSs in rhizobia and plant pathogenic bacteria.Frontiers in plant science · 2014Review
- Distinct cell surface appendages produced by Sinorhizobium fredii USDA257 and S. fredii USDA191, cultivar-specific and nonspecific symbionts of soybean.Applied and environmental microbiology · 2011Article
- Rhizobial measures to evade host defense strategies and endogenous threats to persistent symbiotic nitrogen fixation: a focus on two legume-rhizobium model systems.Cellular and molecular life sciences : CMLS · 2011Review
- Symbiotic use of pathogenic strategies: rhizobial protein secretion systems.Nature reviews. Microbiology · 2009Review
- Symbiosis-promoting and deleterious effects of NopT, a novel type 3 effector of Rhizobium sp. strain NGR234.Journal of bacteriology · 2008Article
- HpaC controls substrate specificity of the Xanthomonas type III secretion system.PLoS pathogens · 2008Article
- TtsI regulates symbiotic genes in Rhizobium species NGR234 by binding to tts boxes.Molecular microbiology · 2008Article
- Identification of protein secretion systems and novel secreted proteins in Rhizobium leguminosarum bv. viciae.BMC genomics · 2008Article
- Protein-protein interactions within type III secretion system-dependent pili of Rhizobium sp. strain NGR234.Journal of bacteriology · 2008Article
- PopF1 and PopF2, two proteins secreted by the type III protein secretion system of Ralstonia solanacearum, are translocators belonging to the HrpF/NopX family.Journal of bacteriology · 2006Article
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhizobium sp. strain NGR234 possesses a functional type three secretion system (TTSS), through which a number of proteins, called nodulation outer proteins (Nops), are delivered to the outside of the cell. A major constraint to the identification of Nops is their low abundance in the supernatants of NGR234 strains grown in culture. To overcome this limitation, a more sensitive proteomics-based strategy was developed. Secreted proteins from wild-type NGR234 were separated by two-dimensional gel electrophoresis, and the gel was compared to similar gels containing the proteins from a TTSS mutant (NGROmegarhcN). To identify the proteins, spots unique to the NGR234 gels were analyzed by matrix-assisted laser desorption ionization-time of flight mass spectrometry and the data were compared to the sequence of the symbiotic plasmid of NGR234. A nonpolar mutant of one of these proteins was generated called NopB. NopB is required for Nop secretion but inhibits the interaction with Pachyrhizus tuberosus and augments nodulation of Tephrosia vogelii. Flavonoids and a functional TTSS are required for the formation of some surface appendages on NGR234. In situ immunogold labeling and isolation of these pili showed that they contain NopB.
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