ArticleJournal of bacteriology2005
Characterization of the RokA and HexA broad-substrate-specificity hexokinases from Bacteroides fragilis and their role in hexose and N-acetylglucosamine utilization.
Article in Journal of bacteriology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 42 citations in OpenAlex.
- Hierarchical glycolytic pathways control the carbohydrate utilization regulator in human gut Bacteroides.Nature communications · 2025Article
- Barcoded overexpression screens in gut Bacteroidales identify genes with roles in carbon utilization and stress resistance.Nature communications · 2024Article
- Changes of gut microbiota reflect the severity of major depressive disorder: a cross sectional study.Translational psychiatry · 2023Article
- The ROK kinase N-acetylglucosamine kinase uses a sequential random enzyme mechanism with successive conformational changes upon each substrate binding.The Journal of biological chemistry · 2023Article
- A Novel Family of RNA-Binding Proteins Regulate Polysaccharide Metabolism inJournal of bacteriology · 2021Article
- A family of anti-Bacteroidales peptide toxins wide-spread in the human gut microbiota.Nature communications · 2019Article
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- Host Sialic Acids: A Delicacy for the Pathogen with Discerning Taste.Microbiology spectrum · 2015Review
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- Functional metabolic map of Faecalibacterium prausnitzii, a beneficial human gut microbe.Journal of bacteriology · 2014Article
- Dynamic responses of Bacteroides thetaiotaomicron during growth on glycan mixtures.Molecular microbiology · 2013Article
- Glucose phosphorylation is required for Mycobacterium tuberculosis persistence in mice.PLoS pathogens · 2013Article
- Sialic acid, periodontal pathogens and Tannerella forsythia: stick around and enjoy the feast!Molecular oral microbiology · 2012Review
- An infant-associated bacterial commensal utilizes breast milk sialyloligosaccharides.The Journal of biological chemistry · 2011Article
- Identification and regulation of the N-acetylglucosamine utilization pathway of the plant pathogenic bacterium Xanthomonas campestris pv. campestris.Journal of bacteriology · 2010Article
- Sialic acid (N-acetyl neuraminic acid) utilization by Bacteroides fragilis requires a novel N-acetyl mannosamine epimerase.Journal of bacteriology · 2009Article
- Characterizing a model human gut microbiota composed of members of its two dominant bacterial phyla.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Bacteroides: the good, the bad, and the nitty-gritty.Clinical microbiology reviews · 2007Review
- Molecular characterization of a glucokinase with broad hexose specificity from Bacillus sphaericus strain C3-41.Applied and environmental microbiology · 2007Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Bacteroides fragilis, a human gastrointestinal commensal and an opportunistic pathogen, utilizes simple and complex sugars and polysaccharides for growth in the large intestine and at sites of infection. Because B. fragilis lacks transport-linked sugar phosphorylation systems, cytoplasmic kinase(s) was expected to be required for the phosphorylation of hexoses and hexosamines. We have now identified two hexose kinases that are important for growth of B. fragilis on glucose, mannose, and other sugars. One kinase (RokA), a member of the ROK family of proteins, was found to be the sole kinase for activation of N-acetyl-D-glucosamine (NAG). The other kinase (HexA) is responsible for the majority of the glucose kinase activity in the cell, although a hexA deletion mutant strain was not defective for growth on any substrate tested. Deletion of both the rokA and hexA kinase genes resulted in inability of the cell to use glucose, mannose, NAG, and many other sugars. We purified RokA and determined its approximate molecular mass to be 36.5 kDa. The purified RokA protein was shown to phosphorylate several substrates, including glucose, NAG, and mannose, but not N-acetylmannosamine or N-acetylneuraminic acid. Phylogenetic analysis of RokA showed that it is most similar to kinases from the Cytophaga-Flavibacterium-Bacteroides group, while HexA was most similar to other bacterial hexokinases and eukaryotic hexokinases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.