ArticleThe Plant cell2005
Plus and minus sexual agglutinins from Chlamydomonas reinhardtii.
Article in The Plant cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
47 citing papers in PubMed, 89 citations in OpenAlex.
- The primary cilium as a multifunctional organelle: emerging roles and unanswered questions.Cell communication and signaling : CCS · 2025Review
- Engineering microalgal cell wall-anchored proteins using GP1 PPSPX motifs and releasing with intein-mediated fusion.bioRxiv : the preprint server for biology · 2025Article
- Extensive remodeling during Chlamydomonas reinhardtii zygote maturation leads to highly resistant zygospores.The Plant journal : for cell and molecular biology · 2025Article
- FromiScience · 2024Article
- Characteristics of NGenomics, proteomics & bioinformatics · 2023Article
- A conserved RWP-RK transcription factor VSR1 controls gametic differentiation in volvocine algae.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Developmental changes in ciliary composition during gametogenesis inMolecular biology of the cell · 2022Article
- Crossing and selection of Chlamydomonas reinhardtii strains for biotechnological glycolate production.Applied microbiology and biotechnology · 2022Article
- Intrinsically Disordered Proteins: Critical Components of the Wetware.Chemical reviews · 2022Review
- Cell-Cell Mating Interactions: Overview and Potential of Single-Cell Force Spectroscopy.International journal of molecular sciences · 2022Review
- MAR1 links membrane adhesion to membrane merger during cell-cell fusion in Chlamydomonas.Developmental cell · 2021Article
- Species-specific gamete recognition initiates fusion-driving trimer formation by conserved fusogen HAP2.Nature communications · 2021Article
- HAP2-Mediated Gamete Fusion: Lessons From the World of Unicellular Eukaryotes.Frontiers in cell and developmental biology · 2021Review
- Transient Internalization and Microtubule-Dependent Trafficking of a Ciliary Signaling Receptor from the Plasma Membrane to the Cilium.Current biology : CB · 2019Article
- Cell wall integrity signaling regulates cell wall-related gene expression in Chlamydomonas reinhardtii.Scientific reports · 2019Article
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- Evaluation, comparison of different solvent extraction, cell disruption methods and hydrothermal liquefaction ofBiotechnology reports (Amsterdam, Netherlands) · 2019Article
- How the Ciliary Membrane Is Organized Inside-Out to Communicate Outside-In.Current biology : CB · 2018Review
- Gene Regulatory Networks for the Haploid-to-Diploid Transition ofPlant physiology · 2017Article
- Insights into the Evolution of Hydroxyproline-Rich Glycoproteins from 1000 Plant Transcriptomes.Plant physiology · 2017Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
Gametes of the unicellular green alga Chlamydomonas reinhardtii undergo sexual adhesion via enormous chimeric Hyp-rich glycoproteins (HRGPs), the plus and minus sexual agglutinins, that are displayed on their flagellar membrane surfaces. We have previously purified the agglutinins and analyzed their structural organization using electron microscopy. We report here the cloning and sequencing of the Sag1 and Sad1 genes that encode the two agglutinins and relate their derived amino acid sequences and predicted secondary structure to the morphology of the purified proteins. Both agglutinin proteins are organized into three distinct domains: a head, a shaft in a polyproline II configuration, and an N-terminal domain. The plus and minus heads are related in overall organization but poorly conserved in sequence except for two regions of predicted hydrophobic alpha-helix. The shafts contain numerous repeats of the PPSPX motif previously identified in Gp1, a cell wall HRGP. We propose that the head domains engage in autolectin associations with the distal termini of their own shafts and suggest ways that adhesion may involve head-head interactions, exolectin interactions between the heads and shafts of opposite type, and antiparallel shaft-shaft interactions mediated by carbohydrates displayed in polyproline II configurations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.