ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2005
Endogenous Ca2+ buffer concentration and Ca2+ microdomains in hippocampal neurons.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 118 citations in OpenAlex.
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- Plasma membrane calcium ATPases and cerebellar pathology: what's the role in the ataxia?Biology direct · 2025Review
- Modeling traveling calcium waves in cellular structures.Journal of computational neuroscience · 2025Article
- Physiology of intracellular calcium buffering.Physiological reviews · 2023Review
- Calbindin-Expressing CA1 Pyramidal Neurons Encode Spatial Information More Efficiently.eNeuro · 2023Article
- Presenilins regulate synaptic plasticity in the perforant pathways of the hippocampus.Molecular brain · 2023Article
- Calcium modeling of spine apparatus-containing human dendritic spines demonstrates an "all-or-nothing" communication switch between the spine head and dendrite.PLoS computational biology · 2022Article
- Influence of T-Bar on Calcium Concentration Impacting Release Probability.Frontiers in computational neuroscience · 2022Article
- Designing AAV Vectors for Monitoring the Subtle Calcium Fluctuations of Inferior Olive NetworkFrontiers in cellular neuroscience · 2022Article
- Current Understanding of the Neural Circuitry in the Comorbidity of Chronic Pain and Anxiety.Neural plasticity · 2022Review
- Mechanism ofEvidence-based complementary and alternative medicine : eCAM · 2021Article
- Posterior basolateral amygdala to ventral hippocampal CA1 drives approach behaviour to exert an anxiolytic effect.Nature communications · 2020Article
- Cytosolic CaCold Spring Harbor perspectives in biology · 2020Review
- A Computational Model of the Cholinergic Modulation of CA1 Pyramidal Cell Activity.Frontiers in computational neuroscience · 2020Article
- Geometric Control of Frequency Modulation of cAMP Oscillations due to Calcium in Dendritic Spines.Biophysical journal · 2019Article
- Cellular and Molecular Differences Between Area CA1 and the Dentate Gyrus of the Hippocampus.Molecular neurobiology · 2019Review
- Calbindin-D28K Limits Dopamine Release in Ventral but Not Dorsal Striatum by Regulating CaACS chemical neuroscience · 2019Article
- Intracellular calcium stores mediate metaplasticity at hippocampal dendritic spines.The Journal of physiology · 2019Article
- Biphasic Modulation of NMDA Receptor Function by Metabotropic Glutamate Receptors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
12 more citing papers are in PubMed but not listed here.
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6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ca2+-binding proteins are ubiquitously expressed throughout the CNS and serve as valuable immunohistochemical markers for certain types of neurons. However, the functional role of most Ca2+-binding proteins has to date remained obscure because their concentration in central neurons is not known. In this study, we investigate the intracellular concentration of the widely expressed Ca2+-binding protein calbindin-D28k in adult hippocampal slices using patch-clamp recordings and immunohistochemistry. First, we show that calbindin-D28k freely exchanges between patch pipette and cytoplasm during whole cell patch-clamp recordings with a time constant of approximately 10 min. Substituting known concentrations of recombinant calbindin-D28k in patch pipettes enabled us to determine the endogenous calbindin-D28k concentration by postrecording immunohistochemistry. Using this calibration procedure, we find that mature granule cells (doublecortin-) contain approximately 40 microm, and newborn granule cells (doublecortin+) contain 0-20 microm calbindin-D28k. CA3 stratum radiatum interneurons and CA1 pyramidal cells enclose approximately 47 and approximately 45 microm calbindin-D28k, respectively. Numerical simulations showed that 40 microm calbindin-D28k is capable of tuning Ca2+ microdomains associated with action potentials at the mouth of single or clustered Ca2+ channels: calbindin-D28k reduces the increment in free Ca2+ at a distance of 100 and 200 nm by 20 and 35%, respectively, and strongly accelerates the collapse of the Ca2+ gradient after cessation of Ca2+ influx. These data suggest that calbindin-D28k equips hippocampal neurons with approximately 160 microm mobile, high-affinity Ca2+-binding sites (kappa(S) approximately 200) that slow and reduce global Ca2+ signals while they enhance the spatiotemporal fidelity of submicroscopic Ca2+ signals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.