ArticleRNA (New York, N.Y.)2005
Number, position, and significance of the pseudouridines in the large subunit ribosomal RNA of Haloarcula marismortui and Deinococcus radiodurans.
Article in RNA (New York, N.Y.), 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 43 citations in OpenAlex.
- Complete list of canonical post-transcriptional modifications in the Bacillus subtilis ribosome and their link to RbgA driven large subunit assembly.Nucleic acids research · 2024Article
- Complete list of canonical post-transcriptional modifications in thebioRxiv : the preprint server for biology · 2024Article
- Landscape of RNA pseudouridylation in archaeon Sulfolobus islandicus.Nucleic acids research · 2024Article
- Naturally occurring modified ribonucleosides.Wiley interdisciplinary reviews. RNA · 2020Review
- Pseudouridine-Free Escherichia coli Ribosomes.Journal of bacteriology · 2018Article
- A mass spectrometry-based method for direct determination of pseudouridine in RNA.Nucleic acids research · 2016Article
- An atlas of RNA base pairs involving modified nucleobases with optimal geometries and accurate energies.Nucleic acids research · 2015Article
- Structure modulation of helix 69 from Escherichia coli 23S ribosomal RNA by pseudouridylations.Nucleic acids research · 2014Article
- Identification and characterization of the Thermus thermophilus 5-methylcytidine (m5C) methyltransferase modifying 23 S ribosomal RNA (rRNA) base C1942.The Journal of biological chemistry · 2012Article
- Discovery of Pyrobaculum small RNA families with atypical pseudouridine guide RNA features.RNA (New York, N.Y.) · 2012Article
- YhiQ is RsmJ, the methyltransferase responsible for methylation of G1516 in 16S rRNA of E. coli.Journal of molecular biology · 2012Article
- Mass spectrometry-based quantification of pseudouridine in RNA.Journal of the American Society for Mass Spectrometry · 2011Article
- Pseudouridine formation in archaeal RNAs: The case of Haloferax volcanii.RNA (New York, N.Y.) · 2011Article
- Inactivation of the RluD pseudouridine synthase has minimal effects on growth and ribosome function in wild-type Escherichia coli and Salmonella enterica.Journal of bacteriology · 2011Article
- An overview of the introns-first theory.Journal of molecular evolution · 2009Article
- Structural basis for cross-resistance to ribosomal PTC antibiotics.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- RNomics and Modomics in the halophilic archaea Haloferax volcanii: identification of RNA modification genes.BMC genomics · 2008Article
- Mass spectrometry of the fifth nucleoside: a review of the identification of pseudouridine in nucleic acids.Analytica chimica acta · 2008Review
- An indigenous posttranscriptional modification in the ribosomal peptidyl transferase center confers resistance to an array of protein synthesis inhibitors.Journal of molecular biology · 2008Article
- Combined in silico and experimental identification of the Pyrococcus abyssi H/ACA sRNAs and their target sites in ribosomal RNAs.Nucleic acids research · 2008Article
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
The number and position of the pseudouridines of Haloarcula marismortui and Deinococcus radiodurans large subunit RNA have been determined by a combination of total nucleoside analysis by HPLC-mass spectrometry and pseudouridine sequencing by the reverse transcriptase method and by LC/MS/MS. Three pseudouridines were found in H. marismortui, located at positions 1956, 1958, and 2621 corresponding to Escherichia coli positions 1915, 1917, and 2586, respectively. The three pseudouridines are all in locations found in other organisms. Previous reports of a larger number of pseudouridines in this organism were incorrect. Three pseudouridines and one 3-methyl pseudouridine (m3Psi) were found in D. radiodurans 23S RNA at positions 1894, 1898 (m3Psi), 1900, and 2584, the m3Psi site being determined by a novel application of mass spectrometry. These positions correspond to E. coli positions 1911, 1915, 1917, and 2605, which are also pseudouridines in E. coli (1915 is m3Psi). The pseudouridines in the helix 69 loop, residues 1911, 1915, and 1917, are in positions highly conserved among all phyla. Pseudouridine 2584 in D. radiodurans is conserved in eubacteria and a chloroplast but is not found in archaea or eukaryotes, whereas pseudouridine 2621 in H. marismortui is more conserved in eukaryotes and is not found in eubacteria. All the pseudoridines are near, but not exactly at, nucleotides directly involved in various aspects of ribosome function. In addition, two D. radiodurans Psi synthases responsible for the four Psi were identified.
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