ArticleMolecular and cellular biology2005
Identification of putative new splicing targets for ETR-3 using sequences identified by systematic evolution of ligands by exponential enrichment.
Article in Molecular and cellular biology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 98 citations in OpenAlex.
- CELF1 represses Doublesex1 expression via its 5' UTR in the crustacean Daphnia magna.PloS one · 2022Article
- Investigating the ACE2 polymorphisms in COVID-19 susceptibility: An in silico analysis.Molecular genetics & genomic medicine · 2021Article
- CELF2 regulates the species-specific alternative splicing of TREM2.Scientific reports · 2020Article
- Alternative splicing regulation of doublesex gene by RNA-binding proteins in the silkworm Bombyx mori.RNA biology · 2019Article
- Myotonic Dystrophy and Developmental Regulation of RNA Processing.Comprehensive Physiology · 2018Review
- Aromatic side-chain conformational switch on the surface of the RNA Recognition Motif enables RNA discrimination.Nature communications · 2017Article
- Activation-Dependent TRAF3 Exon 8 Alternative Splicing Is Controlled by CELF2 and hnRNP C Binding to an Upstream Intronic Element.Molecular and cellular biology · 2017Article
- The alternative splicing program of differentiated smooth muscle cells involves concerted non-productive splicing of post-transcriptional regulators.Nucleic acids research · 2016Article
- Roles for RNA-binding proteins in development and disease.Brain research · 2016Review
- Article
- Position-dependent activity of CELF2 in the regulation of splicing and implications for signal-responsive regulation in T cells.RNA biology · 2016Article
- Identification of Targets of CUG-BP, Elav-Like Family Member 1 (CELF1) Regulation in Embryonic Heart Muscle.PloS one · 2016Article
- Diverse regulation of 3' splice site usage.Cellular and molecular life sciences : CMLS · 2015Review
- Analysis of the preferences for splice codes across tissues.Protein & cell · 2015Article
- Antagonistic regulation of mRNA expression and splicing by CELF and MBNL proteins.Genome research · 2015Article
- Identification of recurrent regulated alternative splicing events across human solid tumors.Nucleic acids research · 2015Article
- Splicing predictions reliably classify different types of alternative splicing.RNA (New York, N.Y.) · 2015Article
- An optogenetic gene expression system with rapid activation and deactivation kinetics.Nature chemical biology · 2014Article
- Purification, crystallization and preliminary crystallographic studies of C-terminal RNA recognition motif (RRM-3) of human ELAV-type RNA-binding protein 3 (ETR-3).Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
- Structural insights into the targeting of mRNA GU-rich elements by the three RRMs of CELF1.Nucleic acids research · 2013Article
3 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 2 institutions in 3 countries.
Funding
Abstract
ETR-3 (also know as BRUNOL3, NAPOR, and CUGBP2) is one of six members of the CELF (CUG-BP1- and ETR-3-like factor) family of splicing regulators. ETR-3 regulates splicing by direct binding to the pre-mRNA. We performed systematic evolution of ligands by exponential enrichment (SELEX) to identify the preferred binding sequence of ETR-3. After five rounds of SELEX, ETR-3 selected UG-rich sequences, in particular UG repeats and UGUU motifs. Either of these selected motifs was able to restore ETR-3 binding and responsiveness to a nonresponsive splicing reporter in vivo. Moreover, this effect was not specific to ETR-3 since minigenes containing either of the two motifs were responsive to two other CELF proteins (CUG-BP1 and CELF4), indicating that different members of the CELF family can mediate their effects via a common binding site. Using the SELEX-identified motifs to search the human genome, we identified several possible new ETR-3 targets. We created minigenes for two of these genes, the CFTR and MTMR1 genes, and confirmed that ETR-3 regulates their splicing patterns. For the CFTR minigene this regulation was demonstrated to be dependent on the presence of the putative binding site identified in our screen. These results validate this approach to search for new targets for RNA processing proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.