ArticleProceedings of the National Academy of Sciences of the United States of America2005
Metabolizing enzyme toxicology assay chip (MetaChip) for high-throughput microscale toxicity analyses.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 161 citations in OpenAlex.
- Recent advances in microarray 3D bioprinting for high-throughput spheroid and tissue culture and analysis.Essays in biochemistry · 2021Review
- Bioactivity Profiling of Small-Volume Samples by Nano Liquid Chromatography Coupled to Microarray Bioassaying Using High-Resolution Fractionation.Analytical chemistry · 2019Article
- Advances in Engineered Human Liver Platforms for Drug Metabolism Studies.Drug metabolism and disposition: the biological fate of chemicals · 2018Article
- Hollow Micropillar Array Method for High-Capacity Drug Screening on Filter-Grown Epithelial Cells.Analytical chemistry · 2018Article
- Prediction of metabolism-induced hepatotoxicity on three-dimensional hepatic cell culture and enzyme microarrays.Archives of toxicology · 2018Article
- Deconvolution of images from 3D printed cells in layers on a chip.Biotechnology progress · 2018Article
- A novel lab-on-a-chip platform for spheroid metabolism monitoring.Cytotechnology · 2018Article
- High-throughput approaches for screening and analysis of cell behaviors.Biomaterials · 2018Review
- Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays.Journal of visualized experiments : JoVE · 2017Article
- Screening applications in drug discovery based on microfluidic technology.Biomicrofluidics · 2016Review
- A versatile snap chip for high-density sub-nanoliter chip-to-chip reagent transfer.Scientific reports · 2015Article
- Manipulating biological agents and cells in micro-scale volumes for applications in medicine.Chemical Society reviews · 2013Review
- Life on a microarray: assessing live cell functions in a microarray format.Cellular and molecular life sciences : CMLS · 2012Review
- Metabolic toxicity screening using electrochemiluminescence arrays coupled with enzyme-DNA biocolloid reactors and liquid chromatography-mass spectrometry.Annual review of analytical chemistry (Palo Alto, Calif.) · 2012Review
- Adaptation of high-throughput screening in drug discovery-toxicological screening tests.International journal of molecular sciences · 2012Review
- Microengineering methods for cell-based microarrays and high-throughput drug-screening applications.Biofabrication · 2011Article
- Drug-eluting microarrays for cell-based screening of chemical-induced apoptosis.Analytical chemistry · 2011Article
- A sandwiched microarray platform for benchtop cell-based high throughput screening.Biomaterials · 2011Article
- Three-dimensional cell culture microarray for high-throughput studies of stem cell fate.Biotechnology and bioengineering · 2010Article
- Metabolic enzyme microarray coupled with miniaturized cell-culture array technology for high-throughput toxicity screening.Methods in molecular biology (Clifton, N.J.) · 2010Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The clinical progression of new chemical entities to pharmaceuticals remains hindered by the relatively slow pace of technology development in toxicology and clinical safety evaluation, particularly in vitro approaches, that can be used in the preclinical and early clinical phases of drug development. To alleviate this bottle-neck, we have developed a metabolizing enzyme toxicology assay chip (MetaChip) that combines high-throughput P450 catalysis with cell-based screening on a microscale platform. The MetaChip concept is demonstrated by using sol-gel encapsulated P450s to activate the prodrug cyclophosphamide, which is the major constituent of the anticancer drug Cytoxan, as well as other compounds that are activated by P450 metabolism. The MetaChip provides a high-throughput microscale alternative to currently used in vitro methods for human metabolism and toxicology screening based on liver slices, cultured human hepatocytes, purified microsomal preparations, or isolated and purified P450s. This technology creates opportunities for rapid and inexpensive assessment of ADME/Tox (absorption, distribution, metabolism, excretion/toxicology) at very early phases of drug development, thereby enabling unsuitable candidates to be eliminated from consideration much earlier in the drug discovery process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.