Evidence map›Paper›PMID 15655666›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2005

Development and mapping of microsatellite (SSR) markers in wheat.

Q J Song, J R Shi, S Singh, E W Fickus, J M Costa, J Lewis, B S Gill, R Ward, P B Cregan

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 210 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
210citing papers in PubMed, 2 pooled it
54.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

210 citing papers in PubMed, 2 syntheses or guidelines pooled it, 633 citations in OpenAlex.

  1. Pooled it
  2. Pooled it
  3. Article
  4. Article
  5. Genetic mapping of QTLs for resistance to bacterial leaf streak in hexaploid wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024
    Article
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  7. Genetic linkage map construction and QTL analysis for plant height in proso millet (Panicum miliaceum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024
    Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Development and utility of SSR markers based onFrontiers in plant science · 2023
    Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Weed competitive ability in wheat: a peek through in its functional significance, present status and future prospects.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021
    Review

150 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Q J SongSoybean Genomics and Improvement Lab, Beltsville Agricultural Research Center, USDA-ARS, MD, 20705, USA.
J R Shi
S Singh
E W Fickus
J M Costa
J Lewis
B S Gill
R Ward
P B Cregan
Michigan State University · USAgricultural Research Service · USKansas State University · USBeltsville Agricultural Research Center · USUniversity of Maryland, College Park · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microsatellite DNA markers are consistently found to be more informative than other classes of markers in hexaploid wheat. The objectives of this research were to develop new primers flanking wheat microsatellites and to position the associated loci on the wheat genome map by genetic linkage mapping in the ITMI W7984 x Opata85 recombinant inbred line (RIL) population and/or by physical mapping with cytogenetic stocks. We observed that the efficiency of marker development could be increased in wheat by creating libraries from sheared rather than enzyme-digested DNA fragments for microsatellite screening, by focusing on microsatellites with the [ATT/TAA]n motif, and by adding an untemplated G-C clamp to the 5'-end of primers. A total of 540 microsatellite-flanking primer pairs were developed, tested, and annotated from random genomic libraries. Primer pairs and associated loci were assigned identifiers prefixed with BARC (the acronym for the USDA-ARS Beltsville Agricultural Research Center) or Xbarc, respectively. A subset of 315 primer sets was used to map 347 loci. One hundred and twenty-five loci were localized by physical mapping alone. Of the 222 loci mapped with the ITMI population, 126 were also physically mapped. Considering all mapped loci, 126, 125, and 96 mapped to the A, B, and D genomes, respectively. Twenty-three of the new loci were positioned in gaps larger than 10 cM in the map based on pre-existing markers, and 14 mapped to the ends of chromosomes. The length of the linkage map was extended by 80.7 cM. Map positions were consistent for 111 of the 126 loci positioned by both genetic and physical mapping. The majority of the 15 discrepancies between genetic and physical mapping involved chromosome group 5.

Indexed as

Chromosome MappingBase SequenceDNA PrimersGene LibraryMicrosatellite RepeatsMolecular Sequence DataSequence AlignmentSequence Analysis, DNATriticumDNA Primers

Identifiers

PMID15655666
OpenAlexW2089109288

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.