ArticleGenetics2005
Gene clusters for insecticidal loline alkaloids in the grass-endophytic fungus Neotyphodium uncinatum.
Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
38 citing papers in PubMed, 149 citations in OpenAlex.
- Microbial unusual gene clusters without prominent core enzymes: natural products, biosynthesis and genome mining.The Journal of antibiotics · 2026Review
- Identification and Characterization of ThreeJournal of fungi (Basel, Switzerland) · 2025Article
- Decarboxylation in Natural Products Biosynthesis.JACS Au · 2024Review
- Discovery and Characterization ofMicroorganisms · 2024Article
- Extracellular Enzyme of Endophytic Fungi Isolated fromInternational journal of biomaterials · 2022Article
- Article
- Molecular insights into the endoperoxide formation by Fe(II)/α-KG-dependent oxygenase NvfI.Nature communications · 2021Article
- Global Changes in AsexualMicroorganisms · 2021Article
- Article
- Contrasting effects of Rhizophagus irregularis versus bacterial and fungal seed endophytes on Trifolium repens plant-soil feedback.Mycorrhiza · 2021Article
- Review
- Molecular mechanisms in grass-Epichloë interactions: towards endophyte driven farming to improve plant fitness and immunity.World journal of microbiology & biotechnology · 2020Review
- Infection Rates and Alkaloid Patterns of Different Grass Species with SystemicApplied and environmental microbiology · 2019Article
- Comparison of Strategies to Overcome Drug Resistance: Learning from Various Kingdoms.Molecules (Basel, Switzerland) · 2018Review
- Article
- Endophytic Epichloë species and their grass hosts: from evolution to applications.Plant molecular biology · 2016Review
- Horizontal acquisition of toxic alkaloid synthesis in a clade of plant associated fungi.Fungal genetics and biology : FG & B · 2016Article
- Biosynthesis and Regulation of Bioprotective Alkaloids in the Gramineae Endophytic Fungi with Implications for Herbivores Deterrents.Current microbiology · 2015Review
- Biodiversity of genes encoding anti-microbial traits within plant associated microbes.Frontiers in plant science · 2015Review
- Sequencing and transcriptional analysis of the biosynthesis gene cluster of abscisic acid-producing Botrytis cinerea.International journal of molecular sciences · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Loline alkaloids are produced by mutualistic fungi symbiotic with grasses, and they protect the host plants from insects. Here we identify in the fungal symbiont, Neotyphodium uncinatum, two homologous gene clusters (LOL-1 and LOL-2) associated with loline-alkaloid production. Nine genes were identified in a 25-kb region of LOL-1 and designated (in order) lolF-1, lolC-1, lolD-1, lolO-1, lolA-1, lolU-1, lolP-1, lolT-1, and lolE-1. LOL-2 contained the homologs lolC-2 through lolE-2 in the same order and orientation. Also identified was lolF-2, but its possible linkage with either cluster was undetermined. Most lol genes were regulated in N. uncinatum and N. coenophialum, and all were expressed concomitantly with loline-alkaloid biosynthesis. A lolC-2 RNA-interference (RNAi) construct was introduced into N. uncinatum, and in two independent transformants, RNAi significantly decreased lolC expression (P < 0.01) and loline-alkaloid accumulation in culture (P < 0.001) compared to vector-only controls, indicating involvement of lolC in biosynthesis of lolines. The predicted LolU protein has a DNA-binding site signature, and the relationships of other lol-gene products indicate that the pathway has evolved from various different primary and secondary biosynthesis pathways.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.