ArticleGenetics2005
Characterization of Mos1-mediated mutagenesis in Caenorhabditis elegans: a method for the rapid identification of mutated genes.
Article in Genetics, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 51 citations in OpenAlex.
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- CRELD1 is an evolutionarily-conserved maturational enhancer of ionotropic acetylcholine receptors.eLife · 2018Article
- Targeted genome engineering inCell & bioscience · 2016Review
- Forward and reverse mutagenesis in C. elegans.WormBook : the online review of C. elegans biology · 2014Review
- Targeted heritable mutation and gene conversion by Cas9-CRISPR in Caenorhabditis elegans.Genetics · 2013Article
- Engineering the Caenorhabditis elegans genome using Cas9-triggered homologous recombination.Nature methods · 2013Article
- Molecular antagonism between X-chromosome and autosome signals determines nematode sex.Genes & development · 2013Article
- Positive modulation of a Cys-loop acetylcholine receptor by an auxiliary transmembrane subunit.Nature neuroscience · 2012Article
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- A secreted complement-control-related protein ensures acetylcholine receptor clustering.Nature · 2009Article
- The NemaGENETAG initiative: large scale transposon insertion gene-tagging in Caenorhabditis elegans.Genetica · 2009Review
- Levamisole resistance resolved at the single-channel level in Caenorhabditis elegans.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2008Article
- The hermes transposon of Musca domestica is an efficient tool for the mutagenesis of Schizosaccharomyces pombe.Genetics · 2007Article
- Genetic suppressors of Caenorhabditis elegans pha-4/FoxA identify the predicted AAA helicase ruvb-1/RuvB.Genetics · 2007Article
- Neither maternal nor zygotic med-1/med-2 genes play a major role in specifying the Caenorhabditis elegans endoderm.Genetics · 2007Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insertional mutagenesis with a heterologous transposon provides a method to rapidly determine the molecular identity of mutated genes. The Drosophila transposon Mos1 can be mobilized to cause mutations in Caenorhabditis elegans (Bessereau et al. 2001); however, the mutagenic rate was initially too low for use in most forward genetic screens. To increase the effectiveness of Mos1-mediated mutagenesis we examined the conditions influencing Mos1 transposition. First, optimal transposition occurs 24 hr after expression of the transposase and is unlikely to occur in differentiated sperm or oocytes. Second, transposition is limited to germ-cell nuclei that contain donor elements, but the transposase enzyme can diffuse throughout the gonad syncytium. Third, silencing of transposition is caused by changes in the donor array that occur over time. Finally, multiple transposition events occur in individual germ cells. By using screening techniques based on these results, Mos1 mutagenicity was increased to within an order of magnitude of chemical mutagens.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.