ArticleBiophysical journal2005
3-D particle tracking in a two-photon microscope: application to the study of molecular dynamics in cells.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
What it found
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Who cites it
102 citing papers in PubMed.
- Mapping Nanoscale Forces and Potentials in Live Cells with Microsecond 3D Single-Particle Tracking.Chemical & biomedical imaging · 2026Article
- Three-dimensional tracking of dynamic structures in living cells using single-plane imaging with focus feedback.Nature protocols · 2026Review
- Perspective: An outlook on fluorescence tracking.The Journal of chemical physics · 2025Article
- Single molecule spectrum dynamics imaging with 3D target-locking tracking.Nature communications · 2025Article
- Statistical analysis of fluorescence intensity transients with Bayesian methods.Science advances · 2025Article
- Live-cell super-resolution microscopy reveals how molecules enter and exit the nucleus.Nature · 2025Article
- MINFLUX achieves molecular resolution with minimal photons.Nature photonics · 2025Article
- Modelling Heterogeneous Anomalous Dynamics of Radiation-Induced Double-Strand Breaks in DNA during Non-Homologous End-Joining Pathway.Entropy (Basel, Switzerland) · 2024Article
- Single-molecule tracking to determine the abundances and stoichiometries of freely-diffusing protein complexes in living cells: Past applications and future prospects.The Journal of chemical physics · 2023Review
- Active-Feedback 3D Single-Molecule Tracking Using a Fast-Responding Galvo Scanning Mirror.The journal of physical chemistry. A · 2023Article
- Information optimization of laser scanning microscopes for real-time feedback-driven single particle tracking.Optics express · 2023Article
- Recent Advances in Single-Molecule Tracking and Imaging Techniques.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Study liquid-liquid phase separation with optical microscopy: A methodology review.APL bioengineering · 2023Review
- Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization.Nature communications · 2023Article
- Single-molecule imaging in the primary cilium.Methods in cell biology · 2023Article
- Combined online Bayesian and windowed estimation of background and signal localization facilitates active-feedback particle tracking in complex environments.The Journal of chemical physics · 2022Article
- Measuring Molecular Diffusion in Dynamic Subcellular Nanostructures by Fast Raster Image Correlation Spectroscopy and 3D Orbital Tracking.International journal of molecular sciences · 2022Article
- Real-Time Feedback-Driven Single-Particle Tracking: A Survey and Perspective.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Following the tracks: How transcription factor binding dynamics control transcription.Biophysical journal · 2022Review
- Multiphoton single-molecule localization by sequential excitation with light minima.Light, science & applications · 2022Article
42 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We developed a method for tracking particles in three dimensions designed for a two-photon microscope, which holds great promise to study cellular processes because of low photodamage, efficient background rejection, and improved depth discrimination. During a standard cycle of the tracking routine (32 ms), the laser beam traces four circular orbits surrounding the particle in two z planes above and below the particle. The radius of the orbits is half of the x,y-width of the point spread function, and the distance between the z planes is the z-width of the point spread function. The z-position is adjusted by moving the objective with a piezoelectric-nanopositioner. The particle position is calculated on the fly from the intensity profile obtained during the cycle, and these coordinates are used to set the scanning center for the next cycle. Applying this method, we were able to follow the motion of 500-nm diameter fluorescent polystyrene microspheres moved by a nanometric stage in either steps of 20-100 nm or sine waves of 0.1-10 microm amplitude with 20 nm precision. We also measured the diffusion coefficient of fluorospheres in glycerol solutions and recovered the values expected according to the Stokes-Einstein relationship for viscosities higher than 3.7 cP. The feasibility of this method for live cell measurements is demonstrated studying the phagocytosis of protein-coated fluorospheres by fibroblasts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.