ArticleBiophysical journal2005
Nano- to microscale dynamics of P-selectin detachment from leukocyte interfaces. II. Tether flow terminated by P-selectin dissociation from PSGL-1.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Cell Membrane Tension Gradients, Membrane Flows, and Cellular Processes.Physiology (Bethesda, Md.) · 2024Review
- Actin-bundling protein L-plastin promotes megakaryocyte rigidity and dampens proplatelet formation.Haematologica · 2024Article
- Membrane Tubulation with a Biomembrane Force Probe.Membranes · 2023Article
- Probing mechanical interaction of immune receptors and cytoskeleton by membrane nanotube extraction.Scientific reports · 2023Article
- Biomembrane force probe (BFP): Design, advancements, and recent applications to live-cell mechanobiology.Exploration (Beijing, China) · 2023Review
- Spatially controlled construction of assembloids using bioprinting.Nature communications · 2023Article
- Inside-out regulation of E-cadherin conformation and adhesion.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Single-molecule imaging and microfluidic platform reveal molecular mechanisms of leukemic cell rolling.Communications biology · 2021Article
- Biomechanics of Neutrophil Tethers.Life (Basel, Switzerland) · 2021Review
- Aspiration-assisted bioprinting for precise positioning of biologics.Science advances · 2020Article
- Endothelial Surface Protrusion by a Point Force.Biophysical journal · 2016Article
- Actin filament turnover drives leading edge growth during myelin sheath formation in the central nervous system.Developmental cell · 2015Article
- The properties of chondrocyte membrane reservoirs and their role in impact-induced cell death.Biophysical journal · 2013Article
- Neutrophil rolling at high shear: flattening, catch bond behavior, tethers and slings.Molecular immunology · 2013Review
- Unexpected membrane dynamics unveiled by membrane nanotube extrusion.Biophysical journal · 2013Article
- Effects of plasma membrane cholesterol level and cytoskeleton F-actin on cell protrusion mechanics.PloS one · 2013Article
- Measuring forces at the leading edge: a force assay for cell motility.Integrative biology : quantitative biosciences from nano to macro · 2013Article
- Tangential tether extraction and spontaneous tether retraction of human neutrophils.Biophysical journal · 2012Article
- Effect of viscoelasticity on the analysis of single-molecule force spectroscopy on live cells.Biophysical journal · 2012Article
- Molecular structure of membrane tethers.Biophysical journal · 2012Article
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Authors and funding
3 authors.
Funding
Abstract
We have used a biomembrane force probe decorated with P-selectin to form point attachments with PSGL-1 receptors on a human neutrophil (PMN) in a calcium-containing medium and then to quantify the forces experienced by the attachment during retraction of the PMN at fixed speed. From first touch to final detachment, the typical force history exhibited the following sequence of events: i), an initial linear-elastic displacement of the PMN surface, ii), an abrupt crossover to viscoplastic flow that signaled membrane separation from the interior cytoskeleton and the beginning of a membrane tether, and iii), the final detachment from the probe tip most often by one precipitous step of P-selectin:PSGL-1 dissociation. Analyzing the initial elastic response and membrane unbinding from the cytoskeleton in our companion article I, we focus in this article on the regime of tether extrusion that nearly always occurred before release of the extracellular adhesion bond at pulling speeds > or =1 microm/s. The force during tether growth appeared to approach a plateau at long times. Examined over a large range of pulling speeds up to 150 microm/s, the plateau force exhibited a significant shear thinning as indicated by a weak power-law dependence on pulling speed, f(infinity) = 60 pN(nu(pull)/microm/s)(0.25). Using this shear-thinning response to describe the viscous element in a nonlinear Maxwell-like fluid model, we show that a weak serial-elastic component with a stiffness of approximately 0.07 pN/nm provides good agreement with the time course of the tether force approach to the plateau under constant pulling speed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.