ArticleBiophysical journal2005
Experimental generation and computational modeling of intracellular pH gradients in cardiac myocytes.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 47 citations in OpenAlex.
- CaBiomolecules · 2025Review
- Article
- Intracellular pH Control by Membrane Transport in Mammalian Cells. Insights Into the Selective Advantages of Functional Redundancy.Frontiers in molecular biosciences · 2022Review
- Nitric oxide modulates cardiomyocyte pH control through a biphasic effect on sodium/hydrogen exchanger-1.Cardiovascular research · 2020Article
- Nuclear proton dynamics and interactions with calcium signaling.Journal of molecular and cellular cardiology · 2016Article
- Intracellular tortuosity underlies slow cAMP diffusion in adult ventricular myocytes.Cardiovascular research · 2016Article
- Na⁺ ions as spatial intracellular messengers for co-ordinating Ca²⁺ signals during pH heterogeneity in cardiomyocytes.Cardiovascular research · 2015Article
- Local pH domains regulate NHE3-mediated Na⁺ reabsorption in the renal proximal tubule.American journal of physiology. Renal physiology · 2014Article
- Pumping Ca2+ up H+ gradients: a Ca2(+)-H+ exchanger without a membrane.The Journal of physiology · 2014Review
- Evidence that protons act as neurotransmitters at vestibular hair cell-calyx afferent synapses.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Facilitation by intracellular carbonic anhydrase of Na+ -HCO3- co-transport but not Na+ / H+ exchange activity in the mammalian ventricular myocyte.The Journal of physiology · 2014Article
- Capturing intracellular pH dynamics by coupling its molecular mechanisms within a fully tractable mathematical model.PloS one · 2014Article
- Presynaptic pH and vesicle fusion in Drosophila larvae neurones.Synapse (New York, N.Y.) · 2013Article
- Activity and distribution of intracellular carbonic anhydrase II and their effects on the transport activity of anion exchanger AE1/SLC4A1.The Journal of physiology · 2013Article
- Computer model of unstirred layer and intracellular pH changes. Determinants of unstirred layer pH.Journal of biological physics · 2013Article
- Coupled Ca2+/H+ transport by cytoplasmic buffers regulates local Ca2+ and H+ ion signaling.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Considering protonation as a posttranslational modification regulating protein structure and function.Annual review of biophysics · 2013Review
- Cytosolic H+ microdomain developed around AE1 during AE1-mediated Cl-/HCO3- exchange.The Journal of physiology · 2011Article
- Modeling calcium waves in cardiac myocytes: importance of calcium diffusion.Frontiers in bioscience (Landmark edition) · 2010Article
- Structure-function-folding relationships and native energy landscape of dynein light chain protein: nuclear magnetic resonance insights.Journal of biosciences · 2009Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
Abstract
It is often assumed that pH(i) is spatially uniform within cells. A double-barreled microperfusion system was used to apply solutions of weak acid (acetic acid, CO(2)) or base (ammonia) to localized regions of an isolated ventricular myocyte (guinea pig). A stable, longitudinal pH(i) gradient (up to 1 pH(i) unit) was observed (using confocal imaging of SNARF-1 fluorescence). Changing the fractional exposure of the cell to weak acid/base altered the gradient, as did changing the concentration and type of weak acid/base applied. A diffusion-reaction computational model accurately simulated this behavior of pH(i). The model assumes that H(i)(+) movement occurs via diffusive shuttling on mobile buffers, with little free H(+) diffusion. The average diffusion constant for mobile buffer was estimated as 33 x 10(-7) cm(2)/s, consistent with an apparent H(i)(+) diffusion coefficient, D(H)(app), of 14.4 x 10(-7) cm(2)/s (at pH(i) 7.07), a value two orders of magnitude lower than for H(+) ions in water but similar to that estimated recently from local acid injection via a cell-attached glass micropipette. We conclude that, because H(i)(+) mobility is so low, an extracellular concentration gradient of permeant weak acid readily induces pH(i) nonuniformity. Similar concentration gradients for weak acid (e.g., CO(2)) occur across border zones during regional myocardial ischemia, raising the possibility of steep pH(i) gradients within the heart under some pathophysiological conditions.
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Registered trials
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