ArticleBiophysical journal2005
Ligand accumulation in autocrine cell cultures.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Quantitative Analysis of the Correlation between Cell Size and Cellular Uptake of Particles.Biophysical journal · 2019Article
- Boundary homogenization for a sphere with an absorbing cap of arbitrary size.The Journal of chemical physics · 2016Article
- Note: Boundary homogenization for a circle with periodic absorbing arcs. Exact expression for the effective trapping rate.The Journal of chemical physics · 2015Article
- Comparison of Efficacy of Endogenous and Exogenous IGF-I in Stimulating Matrix Production in Neonatal and Mature Chondrocytes.Cartilage · 2015Article
- Trapping of diffusing particles by striped cylindrical surfaces. Boundary homogenization approach.The Journal of chemical physics · 2015Article
- Cell surface concentrations and concentration ranges for testing in vitro autocrine loops and small molecules.PloS one · 2012Article
- Quantitative understanding of cell signaling: the importance of membrane organization.Current opinion in biotechnology · 2010Review
- Extended narrow escape problem: boundary homogenization-based analysis.Physical review. E, Statistical, nonlinear, and soft matter physics · 2010Article
- Diffusion in a tube of alternating diameter.Chemical physics · 2010Article
- One-dimensional description of diffusion in a tube of abruptly changing diameter: Boundary homogenization based approach.The Journal of chemical physics · 2009Article
- Computational model and microfluidic platform for the investigation of paracrine and autocrine signaling in mouse embryonic stem cells.Molecular bioSystems · 2009Article
- Multiple mechanisms are responsible for transactivation of the epidermal growth factor receptor in mammary epithelial cells.The Journal of biological chemistry · 2008Article
- An inducible autocrine cascade regulates rat hepatocyte proliferation and apoptosis responses to tumor necrosis factor-alpha.Hepatology (Baltimore, Md.) · 2008Article
- Cell-to-cell communication: time and length scales of ligand internalization in cultures of suspended cells.The Journal of chemical physics · 2008Article
- Simulating non-small cell lung cancer with a multiscale agent-based model.Theoretical biology & medical modelling · 2007Article
- Time and length scales of autocrine signals in three dimensions.Biophysical journal · 2007Article
- Cell surface receptors for signal transduction and ligand transport: a design principles study.PLoS computational biology · 2007Review
- Computational modeling of extracellular mechanotransduction.Biophysical journal · 2006Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell-culture assays are routinely used to analyze autocrine signaling systems, but quantitative experiments are rarely possible. To enable the quantitative design and analysis of experiments with autocrine cells, we develop a biophysical theory of ligand accumulation in cell-culture assays. Our theory predicts the ligand concentration as a function of time and measurable parameters of autocrine cells and cell-culture experiments. The key step of our analysis is the derivation of the survival probability of a single ligand released from the surface of an autocrine cell. An expression for this probability is derived using the boundary homogenization approach and tested by stochastic simulations. We use this expression in the integral balance equations, from which we find the Laplace transform of the ligand concentration. We demonstrate how the theory works by analyzing the autocrine epidermal growth factor receptor system and discuss the extension of our methods to other experiments with cultured autocrine cells.
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