ArticleBiophysical journal2005
Nano- to microscale dynamics of P-selectin detachment from leukocyte interfaces. I. Membrane separation from the cytoskeleton.
Article in Biophysical journal, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
66 citing papers in PubMed.
- Article
- Probing the Dynamic Strength of Biomolecular Interactions with Single-Cell Centrifugation.ACS central science · 2025Article
- High-Throughput Centrifuge Force Microscopy Reveals Dynamic Immune-Cell Avidity at the Single-Cell Level.bioRxiv : the preprint server for biology · 2025Article
- Cell Membrane Tension Gradients, Membrane Flows, and Cellular Processes.Physiology (Bethesda, Md.) · 2024Review
- Actin-bundling protein L-plastin promotes megakaryocyte rigidity and dampens proplatelet formation.Haematologica · 2024Article
- Membrane Tubulation with a Biomembrane Force Probe.Membranes · 2023Article
- Probing mechanical interaction of immune receptors and cytoskeleton by membrane nanotube extraction.Scientific reports · 2023Article
- Biomembrane force probe (BFP): Design, advancements, and recent applications to live-cell mechanobiology.Exploration (Beijing, China) · 2023Review
- A mathematical model of fibrinogen-mediated erythrocyte-erythrocyte adhesion.Communications biology · 2023Article
- Inside-out regulation of E-cadherin conformation and adhesion.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Single-molecule imaging and microfluidic platform reveal molecular mechanisms of leukemic cell rolling.Communications biology · 2021Article
- Biomechanics of Neutrophil Tethers.Life (Basel, Switzerland) · 2021Review
- Review
- A biophysical perspective on receptor-mediated virus entry with a focus on HIV.Biochimica et biophysica acta. Biomembranes · 2020Review
- Dynamic constriction and fission of endoplasmic reticulum membranes by reticulon.Nature communications · 2019Article
- Membrane Organization and Physical Regulation of Lymphocyte Antigen Receptors: A Biophysicist's Perspective.The Journal of membrane biology · 2019Review
- Microfluidics-based super-resolution microscopy enables nanoscopic characterization of blood stem cell rolling.Science advances · 2018Article
- Endothelial Surface Protrusion by a Point Force.Biophysical journal · 2016Article
- A Review of Cell Adhesion Studies for Biomedical and Biological Applications.International journal of molecular sciences · 2015Review
- Transport regulation of two-dimensional receptor-ligand association.Biophysical journal · 2015Article
6 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
We have used a biomembrane force probe decorated with P-selectin to form point attachments with PSGL-1 receptors on a human neutrophil (PMN) in a calcium-containing medium and then to quantify the forces experienced by the attachment during retraction of the PMN at fixed speed. From first touch to final detachment, the typical force history exhibited the following sequence of events: i), an initial linear-elastic displacement of the PMN surface, ii), an abrupt crossover to viscoplastic flow that signaled membrane separation from the interior cytoskeleton and the beginning of a membrane tether, and iii), the final detachment from the probe tip by usually one precipitous step of P-selectin:PSGL-1 dissociation. In this first article I, we focus on the initial elastic response and its termination by membrane separation from the cytoskeleton, initiating tether formation. Quantifying membrane unbinding forces for rates of loading (force/time) in the elastic regime from 240 pN/s to 38,000 pN/s, we discovered that the force distributions agreed well with the theory for kinetically limited failure of a weak bond. The kinetic rate for membrane unbinding was found to increase as an exponential function of the pulling force, characterized by an e-fold scale in force of approximately 17 pN and a preexponential factor, or apparent unstressed off rate, of approximately 1/s. The rheological properties of tether growth subsequent to the membrane unbinding events are presented in a companion article II.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.