Evidence map›Paper›PMID 15650816›Full record

ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2005

Using cDNA and genomic sequences as tools to develop SNP strategies in cassava (Manihot esculenta Crantz).

C Lopez, B Piégu, R Cooke, M Delseny, J Tohme, V Verdier

Abstract readComparative Study
PubMed Publisher
In one paragraph

Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
9.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 72 citations in OpenAlex.

  1. Review
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  6. Review
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  11. Identification, validation and high-throughput genotyping of transcribed gene SNPs in cassava.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012
    Article
  12. Article
  13. Article
  14. Identification, validation and survey of a single nucleotide polymorphism (SNP) associated with pungency in Capsicum spp.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2007
    Article
  15. SNP discovery via 454 transcriptome sequencing.The Plant journal : for cell and molecular biology · 2007
    Article
  16. Article
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  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

C LopezLaboratoire Génome et Développement des Plantes, UMR5096, CNRS-Université de Perpignan-Institut de Recherche pour le Développement, 52 Av Paul Alduy, 66860, Perpignan Cedex, France.
B Piégu
R Cooke
M Delseny
J Tohme
V Verdier
Laboratoire Génome et Développement des Plantes · FRCentre National de la Recherche Scientifique · FRInternational Center for Tropical Agriculture · COUniversité de Perpignan · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Single nucleotide polymorphisms (SNP) are the most abundant type of DNA polymorphism found in animal and plant genomes. They provide an important new source of molecular markers that are useful in genetic mapping, map-based positional cloning, quantitative trait locus mapping and the assessment of genetic distances between individuals. Very little is known on the frequency of SNPs in cassava. We have exploited the recently-developed collection of cassava expressed sequence tags (ESTs) to detect SNPs in the five cultivars of cassava used to generate the sequences. The frequency of intra-cultivar and inter-cultivar SNPs after analysis of 111 contigs was one polymorphism per 905 and one per 1,032 bp, respectively; totaling 1 each 509 bp. We have obtained further information on the frequency of SNPs in six cassava cultivars by analysis of 33 amplicons obtained from 3' EST and BAC end sequences. Overall, about 11 kb of DNA sequence was obtained for each cultivar. A total of 186 SNPs (136 and 50 from ESTs and BAC ends, respectively) were identified. Among these, 146 were intra-cultivar polymorphisms, while 80 were inter-cultivar polymorphisms. Thus the total frequency of SNPs was one per 62 bp. This information will help to develop new strategies for EST mapping as well as their association with phenotypic characteristics.

Indexed as

Expressed Sequence TagsPolymorphism, Single NucleotideComputational BiologyManihotSequence Analysis, DNASpecies Specificity

Identifiers

PMID15650816
OpenAlexW2060868842

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.