ArticleJournal of virology2005
Conditional mutations in the mitotic chromosome binding function of the bovine papillomavirus type 1 E2 protein.
Article in Journal of virology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Cottontail Rabbit Papillomavirus E1 and E2 Proteins Mutually Influence Their Subcellular Localizations.Journal of virology · 2018Article
- Replication-dependent and independent mechanisms for the chromosome-coupled persistence of a selfish genome.Nucleic acids research · 2016Article
- Phosphorylation of HPV-16 E2 at serine 243 enables binding to Brd4 and mitotic chromosomes.PloS one · 2014Article
- The papillomavirus E2 proteins.Virology · 2013Review
- Effective formation of the segregation-competent complex determines successful partitioning of the bovine papillomavirus genome during cell division.Journal of virology · 2010Article
- The human papillomavirus type 8 E2 tethering protein targets the ribosomal DNA loci of host mitotic chromosomes.Journal of virology · 2009Article
- Replication and partitioning of papillomavirus genomes.Advances in virus research · 2008Review
- Dynamic localization of the human papillomavirus type 11 origin binding protein E2 through mitosis while in association with the spindle apparatus.Journal of virology · 2006Article
- Bromodomain protein 4 mediates the papillomavirus E2 transcriptional activation function.Journal of virology · 2006Article
- Variations in the association of papillomavirus E2 proteins with mitotic chromosomes.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Inhibition of E2 binding to Brd4 enhances viral genome loss and phenotypic reversion of bovine papillomavirus-transformed cells.Journal of virology · 2005Article
- The mitotic chromosome binding activity of the papillomavirus E2 protein correlates with interaction with the cellular chromosomal protein, Brd4.Journal of virology · 2005Article
- Reconstitution of papillomavirus E2-mediated plasmid maintenance in Saccharomyces cerevisiae by the Brd4 bromodomain protein.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The papillomavirus E2 protein is required for viral transcriptional regulation, DNA replication and genome segregation. We have previously shown that the E2 transactivator protein and BPV1 genomes are associated with mitotic chromosomes; E2 links the genomes to cellular chromosomes to ensure efficient segregation to daughter nuclei. The transactivation domain of the E2 protein is necessary and sufficient for association of the E2 protein with mitotic chromosomes. To determine which residues of this 200-amino-acid domain are important for chromosomal interaction, E2 proteins with amino acid substitutions in each conserved residue of the transactivation domain were tested for their ability to associate with mitotic chromosomes. Chromatin binding was assessed by using immunofluorescence on both spread and directly fixed mitotic chromosomes. E2 proteins defective in the transactivation and replication functions were unable to associate with chromosomes, and those that were competent in these functions were attached to mitotic chromosomes. However, several mutated proteins that were defective for chromosomal interaction could associate with chromosomes after treatment with agents that promote protein folding or when cells were incubated at lower temperatures. These results indicate that precise folding of the E2 transactivation domain is crucial for its interaction with mitotic chromosomes and that this association can be modulated.
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