Evidence map›Paper›PMID 15649648›Full record

ArticleFree radical biology & medicine2005

4-Hydroxynonenal increases gamma-glutamyl transpeptidase gene expression through mitogen-activated protein kinase pathways.

Hongqiao Zhang, Dale A Dickinson, Rui-Ming Liu, Henry Jay Forman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Free radical biology & medicine, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
3.1field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 38 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Redox signaling: An evolution from free radicals to aging.Free radical biology & medicine · 2016
    Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Redox regulation of gamma-glutamyl transpeptidase.American journal of respiratory cell and molecular biology · 2009
    Review
  11. Article
  12. Article
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Hongqiao ZhangDepartment of Environmental Health Science, School of Public Health, University of Alabama at Birmingham, Birmingham, AL 35294, USA.
Dale A Dickinson
Rui-Ming Liu
Henry Jay Forman
University of Alabama at Birmingham · USUniversity of California, Merced · US

Funding

REGULATION OF GLUTATHIONE SYNTHESIS IN OXIDATIVE STRESSR01ES005511 · NIEHS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI FORMAN, HENRY JAY · 1991 to 2010
$2.9M
NIEHS NIH HHS R01 ES005511
6 · The paper itself

Abstract

gamma-Glutamyl transpeptidase (GGT) plays key roles in the metabolism of glutathione. Previous studies have shown that GGT expression was increased by oxidants, but the mechanism remains unclear. In the present study, the effects of 4-hydroxy-2-nonenal (HNE), an electrophilic end product of lipid peroxidation, on GGT expression were investigated in rat lung epithelial type II (L2) cells. We demonstrated that HNE increased GGT activity and mRNA content in both time- and dose-dependent manners. Actinomycin D, an RNA transcription inhibitor, blocked HNE-stimulated increase in GGT mRNA, suggesting transcriptional regulation of GGT mRNA by HNE. Of the seven GGT mRNA transcripts known to be produced from the single rat GGT gene, we found that types I, II, and V-2 were constitutively expressed in L2 cells, but only types I and V-2 were increased by HNE. PD98059 and SB203580, relatively specific inhibitors of the ERK and the p38MAPK kinase pathway, respectively, significantly attenuated HNE induction of both GGT activity and mRNA content. In contrast, studies with JNK inhibitor I, a cell-permeable peptide, indicated that JNK was not involved in the GGT induction by HNE. We also found that GGT induction by HNE could be completely blocked by a cocktail of PD98059 and SB203580, suggesting a combined effect of ERK and p38MAPK pathways in HNE-mediated GGT induction. In conclusion, our results demonstrate that HNE increased GGT expression in rat alveolar type II cells and that the induction of GGT by HNE was mediated through activation of the ERK and p38MAPK pathways.

Indexed as

AldehydesAnimalsCell LineExtracellular Signal-Regulated MAP Kinasesgamma-GlutamyltransferaseGene Expression RegulationMAP Kinase Signaling Systemp38 Mitogen-Activated Protein KinasesProtein Kinase InhibitorsRatsRNA, Messenger4-hydroxy-2-nonenalAldehydesExtracellular Signal-Regulated MAP Kinasesgamma-Glutamyltransferasep38 Mitogen-Activated Protein KinasesProtein Kinase InhibitorsRNA, Messenger

Identifiers

PMID15649648
PMCPMC2801023
OpenAlexW2071244567

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.