ArticlePlanta2005
Arabidopsis emb175 and other ppr knockout mutants reveal essential roles for pentatricopeptide repeat (PPR) proteins in plant embryogenesis.
Article in Planta, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers, 1 of them a synthesis that pooled it.
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Who cites it
74 citing papers in PubMed, 1 synthesis or guideline pooled it, 143 citations in OpenAlex.
- Meta-analysis of grain yield QTL identified during agricultural drought in grasses showed consensus.BMC genomics · 2011Pooled it
- Conserved DNA architect couples chloroplast development to cell cycle in developing cotyledons.Nature plants · 2026Article
- Physiological and molecular response of soybean to combined drought and heat stresses during flowering.BMC plant biology · 2026Article
- Multiomic insights into sucrose accumulation in sugarcane.Plant molecular biology · 2025Article
- CmPPR4 gene controls drought resilience in melon ecotypes.Plant biotechnology journal · 2025Article
- Combined BSA-Seq and RNA-Seq to Identify Potential Genes Regulating Fruit Size in Bottle Gourd (Plants (Basel, Switzerland) · 2024Article
- Clpf encodes pentatricopeptide repeat protein (PPR5) and regulates pink flesh color in watermelon (Citrullus lanatus L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- The dicot homolog of maize PPR103 carries a C-terminal DYW domain and may have a role in C-to-U editing of some chloroplast RNA transcripts.Plant molecular biology · 2024Article
- Sensory plastid-associated PsbP DOMAIN-CONTAINING PROTEIN 3 triggers plant growth- and defense-related epigenetic responses.The Plant journal : for cell and molecular biology · 2023Article
- Characterization of theGenes · 2023Article
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- OsPPR939, a nad5 splicing factor, is essential for plant growth and pollen development in rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- A Pipeline towards the Biochemical Characterization of theInternational journal of molecular sciences · 2021Article
- Research Progress of PPR Proteins in RNA Editing, Stress Response, Plant Growth and Development.Frontiers in genetics · 2021Review
- The Effect of Mutations in the TPR and Ankyrin Families of Alpha Solenoid Repeat Proteins.Frontiers in bioinformatics · 2021Review
- A Genome-Wide Analysis of theGenes · 2020Article
- Chloroplast-to-nucleus retrograde signalling controls intercellular trafficking via plasmodesmata formation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- Characterization of cytoplasmic female sterility in an alloplasmic and monosomic addition line ofBreeding science · 2020Article
- Fine mapping and candidate gene analysis of qhkw5-3, a major QTL for kernel weight in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Transcriptome Landscape Variation in the GenusGenes · 2019Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pentatricopeptide repeat proteins (PPRPs) constitute one of the largest superfamilies in plants, with more than 440 identified in the Arabidopsis thaliana (L.) Heynh genome. While some PPRPs are known to take part in organelle gene expression, little is known about the broader biological contexts of PPRP gene function. Here, using developmental- and reverse-genetic approaches, we demonstrate that a number of PPRPs are essential early in plant development. We have characterized the Arabidopsis embryo-defective175 mutant and identified the EMB175 gene. Emb175 consistently displays aberrant cell organization and undergoes morphological arrest before the globular-heart transition. The emb175 mutation disrupts an intronless open reading frame encoding a predicted chloroplast-localized PPR protein- the first to be rigorously associated with an early embryo-lethal phenotype. To determine if other PPRP genes act in embryogenesis, we searched Arabidopsis insertion mutant collections for pprp knockout alleles, and identified 29 mutants representing 11 loci potentially associated with embryo-defective phenotypes. We assessed gene structures, T-DNA insertion position, and allelism for these loci and were able to firmly establish essential functions for six PPRP genes in addition to EMB175. Interestingly, Nomarski DIC microscopy revealed diverse embryonic defects in these lines, ranging from early lethality to dramatic late-stage morphological defects such as enlarged shoot apices and stunted cotyledons. Together, emb175 and these pprp knockout mutants establish essential roles for PPRPs in embryogenesis, thus broadening the known organismal context for PPRP gene function. The diversity of emb-pprp knockout phenotypes indicates that mutation of different PPRPs can, directly or indirectly, have distinct impacts on embryo morphogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.