ArticlePlanta2005
Molecular cloning, recombinant gene expression, and antifungal activity of cystatin from taro (Colocasia esculenta cv. Kaosiung no. 1).
Article in Planta, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 92 citations in OpenAlex.
- Article
- X-Ray Film Gel Contact Print Method for Analyzing Differential Expression of Plant Protease Inhibitors Under Abiotic Stress.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Functional profile and encapsulating properties of Colocasia esculenta (Taro).Food science & nutrition · 2023Review
- Assessment of ethnobotanical uses, household, and regional genetic diversity of aroid species grown in northeastern India.Frontiers in nutrition · 2023Article
- Oral Microbiome UsingMedicina (Kaunas, Lithuania) · 2022Article
- Label-free quantitative proteomics of maize roots from different root zones provides insight into proteins associated with enhance water uptake.BMC genomics · 2022Article
- Article
- Differential response of silencing HvIcy2 barley plants against Magnaporthe oryzae infection and light deprivation.BMC plant biology · 2018Article
- The wheat multidomain cystatin TaMDC1 displays antifungal, antibacterial, and insecticidal activities in planta.Plant cell reports · 2018Article
- Article
- Rice bifunctional phytocystatin is a dual modulator of legumain and papain-like proteases.Plant molecular biology · 2016Article
- De Novo Assembled Wheat Transcriptomes Delineate Differentially Expressed Host Genes in Response to Leaf Rust Infection.PloS one · 2016Article
- Article
- Beneficial 'unintended effects' of a cereal cystatin in transgenic lines of potato, Solanum tuberosum.BMC plant biology · 2012Article
- Solution structure of a phytocystatin from Ananas comosus and its molecular interaction with papain.PloS one · 2012Article
- Crystal structure of tarocystatin-papain complex: implications for the inhibition property of group-2 phytocystatins.Planta · 2011Article
- Jasmonate signal induced expression of cystatin genes for providing resistance against Karnal bunt in wheat.Plant signaling & behavior · 2011Article
- Article
- In search of decoy/guardee to R genes: deciphering the role of sugars in defense against Fusarium wilt in chickpea.Plant signaling & behavior · 2010Review
- Distinct expression patterns of two Arabidopsis phytocystatin genes, AtCYS1 and AtCYS2, during development and abiotic stresses.Plant cell reports · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A cDNA clone, designated CeCPI, encoding a novel phytocystatin was isolated from taro corms (Colocasia esculenta) using both degenerated primers/RT-PCR amplification and 5'-/3'-RACE extension. The full-length cDNA gene is 1,008 bp in size, encodes 206 amino acid residues, with a deduced molecular weight of 29 kDa. It contains a conserved reactive site motif Gln-Val-Val-Ser-Gly of cysteine protease inhibitors, and another consensus ARFAV sequence for phytocystatin. Sequence analysis revealed that CeCPI is phylogenetically closely related to Eudicots rather than to Monocots, despite taro belonging to Monocot. Recombinant GST-CeCPI fusion protein was overexpressed in Escherichia coli and its inhibitory activity against papain was identified on gelatin/SDS-PAGE. These results confirmed that recombinant CeCPI protein exhibited strong cysteine protease inhibitory activity. Investigation of its antifungal activity clearly revealed a toxic effect on the mycelium growth of phytopathogenic fungi, such as Sclerotium rolfsii Sacc. etc., at a concentration of 80 microg recombinant CeCPI/ ml. Moreover, mycelium growth was completely inhibited and the sclerotia lysed at a concentration of 150-200 microg/ml. Further studies have demonstrated that recombinant CeCPI is capable of acting against the endogenous cysteine proteinase in the fungal mycelium.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.