ArticleDiabetologia2004
Correlation of syntaxin-1 and SNAP-25 clusters with docking and fusion of insulin granules analysed by total internal reflection fluorescence microscopy.
Article in Diabetologia, 2004. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 69 citations in OpenAlex.
- Advanced Imaging Techniques for the Characterization of Subcellular Organelle Structure in Pancreatic Islet β Cells.Comprehensive Physiology · 2023Article
- Cholesterol Redistribution in Pancreatic β-Cells: A Flexible Path to Regulate Insulin Secretion.Biomolecules · 2023Review
- The changing view of insulin granule mobility: From conveyor belt to signaling hub.Frontiers in endocrinology · 2022Review
- Proteomic and Bioinformatic Analysis of Decellularized Pancreatic Extracellular Matrices.Molecules (Basel, Switzerland) · 2021Article
- Dynamin deficiency causes insulin secretion failure and hyperglycemia.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- A glucose-dependent spatial patterning of exocytosis in human β-cells is disrupted in type 2 diabetes.JCI insight · 2019Article
- In situ graphene liquid cell-transmission electron microscopy study of insulin secretion in pancreatic islet cells.International journal of nanomedicine · 2019Article
- MiR-335 overexpression impairs insulin secretion through defective priming of insulin vesicles.Physiological reports · 2017Article
- Evidence of Presynaptic Localization and Function of the c-Jun N-Terminal Kinase.Neural plasticity · 2017Article
- Pancreatic β cell enhancers regulate rhythmic transcription of genes controlling insulin secretion.Science (New York, N.Y.) · 2015Article
- Membrane flexibility, free fatty acids, and the onset of vascular and neurological lesions in type 2 diabetes.Journal of diabetes and metabolic disorders · 2015Review
- Organization and dynamics of SNARE proteins in the presynaptic membrane.Frontiers in physiology · 2015Review
- Review
- Cholesterol-enriched membrane rafts and insulin secretion.Journal of diabetes investigation · 2012Review
- Molecular mechanism of cholesterol- and polyphosphoinositide-mediated syntaxin clustering.Biochemistry · 2011Article
- The organization of the secretory machinery in chromaffin cells as a major factor in modeling exocytosis.HFSP journal · 2010Article
- Evaluation of the heterogeneous reactivity of the syntaxin molecules on the inner leaflet of the plasma membrane.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Expression of neurexin, neuroligin, and their cytoplasmic binding partners in the pancreatic beta-cells and the involvement of neuroligin in insulin secretion.Endocrinology · 2008Article
- Ca2+-secretion coupling is impaired in diabetic Goto Kakizaki rats.The Journal of general physiology · 2007Article
- Alternative splicing of SNAP-25 regulates secretion through nonconservative substitutions in the SNARE domain.Molecular biology of the cell · 2005Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisThe interaction of syntaxin-1 and SNAP-25 with insulin exocytosis was examined using the diabetic Goto-Kakizaki (GK) rat and a total internal reflection fluorescence (TIRF) imaging system.
methodsPrimary rat pancreatic beta cells were immunostained with anti-syntaxin-1A, anti-SNAP-25 and anti-insulin antibodies, and then observed by TIRF microscopy. The real-time image of GFP-labelled insulin granules motion was monitored by TIRF.
resultsThe number of syntaxin-1A and SNAP-25 clusters, and the number of docked insulin granules on the plasma membrane were reduced in GK beta cells. When GK rats were treated with daily insulin injection for 2 weeks, the number of syntaxin-1 and SNAP-25 clusters was restored, along with the number of docked insulin granules. The infection of GK beta cells with Adex1CA SNAP-25 increased the number of docked insulin granules. TIRF imaging analysis demonstrated that the decreased number of fusion events from previously docked insulin granules in GK beta cells was restored when the number of docked insulin granules increased by insulin treatment or Adex1CA SNAP-25 infection. CONCLUSIONS/
interpretationThere was a close correlation between the number of syntaxin-1 and SNAP-25 clusters and the number of docked insulin granules, which is associated with the fusion of insulin granules.
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