ArticleMolecular biology of the cell2005
p27Kip1 acts downstream of N-cadherin-mediated cell adhesion to promote myogenesis beyond cell cycle regulation.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 59 citations in OpenAlex.
- p27Cells · 2025Article
- The Variation in the Traits Ameliorated by Inhibitors of JAK1/2, TGF-β, P-Selectin, and CXCR1/CXCR2 in theInternational journal of molecular sciences · 2024Article
- C-terminally phosphorylated p27 activates self-renewal driver genes to program cancer stem cell expansion, mammary hyperplasia and cancer.Nature communications · 2024Article
- Restoring the Cell Cycle and Proliferation Competence in Terminally Differentiated Skeletal Muscle Myotubes.Cells · 2021Review
- Constricted migration modulates stem cell differentiation.Molecular biology of the cell · 2019Article
- p27 transcriptionally coregulates cJun to drive programs of tumor progression.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Expression of MyoD, insulin like growth factor binding protein, thioredoxin and p27 in secondarily overacting inferior oblique muscles with superior oblique palsy.BMC ophthalmology · 2018Article
- MicroRNA-222 regulates muscle alternative splicing through Rbm24 during differentiation of skeletal muscle cells.Cell death & disease · 2016Article
- Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging.Scientific reports · 2015Article
- PW1/Peg3 expression regulates key properties that determine mesoangioblast stem cell competence.Nature communications · 2015Article
- Early forming label-retaining muscle stem cells require p27kip1 for maintenance of the primitive state.Development (Cambridge, England) · 2014Article
- The influence of high glucose and high insulin on mechanisms controlling cell cycle progression and arrest in mouse C2C12 myoblasts: the comparison with IGF-I effect.Journal of endocrinological investigation · 2014Article
- Cyclin D1 is a major target of miR-206 in cell differentiation and transformation.Cell cycle (Georgetown, Tex.) · 2013Article
- The cell cycle inhibitor p27Kip¹ controls self-renewal and pluripotency of human embryonic stem cells by regulating the cell cycle, Brachyury and Twist.Cell cycle (Georgetown, Tex.) · 2011Article
- Identification of differentially regulated secretome components during skeletal myogenesis.Molecular & cellular proteomics : MCP · 2011Article
- Integrins and cadherins join forces to form adhesive networks.Journal of cell science · 2011Review
- Cdkn1c drives muscle differentiation through a positive feedback loop with Myod.Developmental biology · 2011Article
- MicroRNAs in skeletal myogenesis.Cell cycle (Georgetown, Tex.) · 2011Review
- Secretome Analysis of Skeletal Myogenesis Using SILAC and Shotgun Proteomics.International journal of proteomics · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is widely acknowledged that cultured myoblasts can not differentiate at very low density. Here we analyzed the mechanism through which cell density influences myogenic differentiation in vitro. By comparing the behavior of C2C12 myoblasts at opposite cell densities, we found that, when cells are sparse, failure to undergo terminal differentiation is independent from cell cycle control and reflects the lack of p27Kip1 and MyoD in proliferating myoblasts. We show that inhibition of p27Kip1 expression impairs C2C12 cell differentiation at high density, while exogenous p27Kip1 allows low-density cultured C2C12 cells to enter the differentiative program by regulating MyoD levels in undifferentiated myoblasts. We also demonstrate that the early induction of p27Kip1 is a critical step of the N-cadherin-dependent signaling involved in myogenesis. Overall, our data support an active role of p27Kip1 in the decision of myoblasts to commit to terminal differentiation, distinct from the regulation of cell proliferation, and identify a pathway that, reasonably, operates in vivo during myogenesis and might be part of the phenomenon known as "community effect".
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.