ArticleMolecular biology of the cell2005
Immortalization of human fetal cells: the life span of umbilical cord blood-derived cells can be prolonged without manipulating p16INK4a/RB braking pathway.
Article in Molecular biology of the cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 86 citations in OpenAlex.
- Strategies for immortalisation of amnion-derived mesenchymal and epithelial cells.Cell biology international · 2022Review
- Puromycin-based purification of cells with high expression of the cytochrome P450 CYP3A4 gene from a patient with drug-induced liver injury (DILI).Stem cell research & therapy · 2022Article
- Prospects for the therapeutic development of umbilical cord blood-derived mesenchymal stem cells.World journal of stem cells · 2020Review
- Aging of mesenchymal stem cells: Implication in regenerative medicine.Regenerative therapy · 2018Article
- Cancer-secreted hsa-miR-940 induces an osteoblastic phenotype in the bone metastatic microenvironment via targeting ARHGAP1 and FAM134A.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Can Human Embryonic Stem Cell-Derived Stromal Cells Serve a Starting Material for Myoblasts?Stem cells international · 2017Article
- Label-free assessment of replicative senescence in mesenchymal stem cells by Raman microspectroscopy.Biomedical optics express · 2015Article
- Runx1 and Runx3 Are Downstream Effectors of Nanog in Promoting Osteogenic Differentiation of the Mouse Mesenchymal Cell Line C3H10T1/2.Cellular reprogramming · 2015Article
- Article
- PCB126 inhibits adipogenesis of human preadipocytes.Toxicology in vitro : an international journal published in association with BIBRA · 2015Article
- Differentiation of mesenchymal stem cells into gonad and adrenal steroidogenic cells.World journal of stem cells · 2014Review
- MePR: a novel human mesenchymal progenitor model with characteristics of pluripotency.Stem cells and development · 2013Article
- Amniotic membrane covering for facial nerve repair.Neural regeneration research · 2013Article
- Establishment and characterization of immortalized human amniotic epithelial cells.Cellular reprogramming · 2013Article
- Salient features of mesenchymal stem cells-implications for Ewing sarcoma modeling.Frontiers in oncology · 2013Article
- Culturing on Wharton's jelly extract delays mesenchymal stem cell senescence through p53 and p16INK4a/pRb pathways.PloS one · 2013Article
- Replicative senescence of human bone marrow and umbilical cord derived mesenchymal stem cells and their differentiation to adipocytes and osteoblasts.Molecular biology reports · 2011Article
- The HPB-AML-I cell line possesses the properties of mesenchymal stem cells.Journal of experimental & clinical cancer research : CR · 2010Article
- Mesenchymal stromal cells from human perinatal tissues: From biology to cell therapy.World journal of stem cells · 2010Article
- The Oncogenic Potential of Mesenchymal Stem Cells in the Treatment of Cancer: Directions for Future Research.Current immunology reviews · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human umbilical cord blood-derived mesenchymal stem cells (UCBMSCs) are expected to serve as an excellent alternative to bone marrow-derived human mesenchymal stem cells. However, it is difficult to study them because of their limited life span. To overcome this problem, we attempted to produce a strain of UCBMSCs with a long life span and to investigate whether the strain could maintain phenotypes in vitro. UCBMSCs were infected with retrovirus carrying the human telomerase reverse transcriptase (hTERT) to prolong their life span. The UCBMSCs underwent 30 population doublings (PDs) and stopped dividing at PD 37. The UCBMSCs newly established with hTERT (UCBTERTs) proliferated for >120 PDs. The p16INK4a/RB braking pathway leading to senescence can be inhibited by introduction of Bmi-1, a polycomb-group gene, and human papillomavirus type 16 E7, but the extension of the life span of the UCBMSCs with hTERT did not require inhibition of the p16INK4a/RB pathway. The characteristics of the UCBTERTs remained unchanged during the prolongation of life span. UCBTERTs provide a powerful model for further study of cellular senescence and for future application to cell-based therapy by using umbilical cord blood cells.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.