ArticleProceedings of the National Academy of Sciences of the United States of America2005
Expression of cellulose synthase-like (Csl) genes in insect cells reveals that CslA family members encode mannan synthases.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 159 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
159 citing papers in PubMed, 371 citations in OpenAlex.
- Co-expression of AkCSLA3 and AkMSR1 correlates with altered free monosaccharide pools in Pichia pastoris.BMC plant biology · 2026Article
- Article
- Atypical endo-β-1,4-mannanases are necessary for normal glucomannan synthesis in Arabidopsis.Plant physiology · 2026Article
- Article
- Biased retention and functional diversification of CESA superfamily genes in Medicago mediating development and abiotic stress responses.BMC plant biology · 2026Article
- Unraveling mannan biosynthesis in poplar and spruce: functional characterization of Cellulose Synthase-Like family A (CSLA) genes.The New phytologist · 2026Article
- Genome-Wide Analysis of Cellulose Synthase Superfamily and Roles ofPlants (Basel, Switzerland) · 2025Article
- Cell wall-related glycosyltransferases and wall architecture in the model liverwort Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2025Article
- Illuminating Glucomannan Synthases To Explore Cell Wall Synthesis Bottlenecks.ACS synthetic biology · 2025Article
- Mucilicious methods: Navigating the tools developed to Arabidopsis Seed Coat Mucilage analysis.Cell surface (Amsterdam, Netherlands) · 2025Review
- Genome-wide identification and expression analysis of theFrontiers in plant science · 2025Article
- Review
- Bio-organic fertilizer affects secondary cell wall biosynthesis of Dendrocalamus farinosus by inhibiting the phenylpropanoid metabolic pathway.BMC plant biology · 2024Article
- Genome-Wide Analysis ofInternational journal of molecular sciences · 2024Article
- Genome-wide analysis of the Amorphophallus konjac AkCSLA gene family and its functional characterization in drought tolerance of transgenic arabidopsis.BMC plant biology · 2024Article
- The plant cell wall-dynamic, strong, and adaptable-is a natural shapeshifter.The Plant cell · 2024Review
- Cytochemical localization and synthesis mechanism of the glucomannan in pseudobulbs ofHorticulture research · 2024Article
- CslA and GlxA fromApplied and environmental microbiology · 2024Article
- Article
- Recent Advances in Biomedical Applications of Mannans and Xylans.Current drug targets · 2024Review
99 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glucuronoarabinoxylan, xyloglucan, and galactomannan are noncellulosic polysaccharides found in plant cell walls. All consist of beta-linked glycan backbones substituted with sugar side chains. Although considerable progress has been made in characterizing the structure of these polysaccharides, little is known about the biosynthetic enzymes that produce them. Cellulose synthase-like (Csl) genes are hypothesized to encode Golgi-localized beta-glycan synthases that polymerize the backbones of noncellulosic polysaccharides. To investigate this hypothesis, we used heterologous expression in Drosophila Schneider 2 (S2) cells to systematically analyze the functions of the gene products of a group of Csl genes from Arabidopsis and rice (Oryza sativa L.), including members from five Csl gene families (CslA, CslC, CslD, CslE, and CslH). Our analyses indicate that several members of the CslA gene family encode beta-mannan synthases. Recombinant CslA proteins produce beta-linked mannan polymers when supplied GDP-mannose. The same proteins can produce beta-linked glucomannan heteropolymers when supplied both GDP-mannose and GDP-glucose. One CslA protein also produced beta-linked glucan polymers when supplied GDP-glucose alone. Heterologous expression studies of additional candidate glycan synthases in insect cells or other systems may help identify other noncellulosic polysaccharide biosynthetic enzymes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.