ArticleBritish journal of pharmacology2005
Substrate specificity and functional characterisation of the H+/amino acid transporter rat PAT2 (Slc36a2).
Article in British journal of pharmacology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 37 citations in OpenAlex.
- The Role of Proton-Coupled Amino Acid Transporter 2 (SLC36A2) in Cold-Induced Thermogenesis of Mice.Nutrients · 2023Article
- Reshaping the Binding Pocket of the Neurotransmitter:Solute Symporter (NSS) Family Transporter SLC6A14 (ATBBiomolecules · 2022Article
- PAT2 regulates vATPase assembly and lysosomal acidification in brown adipocytes.Molecular metabolism · 2022Article
- Proline metabolism and transport in retinal health and disease.Amino acids · 2021Review
- Trading amino acids at the aphid-Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Genome-Wide Association Studies of Metabolites in Patients with CKD Identify Multiple Loci and Illuminate Tubular Transport Mechanisms.Journal of the American Society of Nephrology : JASN · 2018Article
- Resculpting the binding pocket of APC superfamily LeuT-fold amino acid transporters.Cellular and molecular life sciences : CMLS · 2018Article
- The Concise Guide to PHARMACOLOGY 2013/14: transporters.British journal of pharmacology · 2013Article
- The SLC36 family of proton-coupled amino acid transporters and their potential role in drug transport.British journal of pharmacology · 2011Review
- SLC36A4 (hPAT4) is a high affinity amino acid transporter when expressed in Xenopus laevis oocytes.The Journal of biological chemistry · 2011Article
- Amino acid derivatives are substrates or non-transported inhibitors of the amino acid transporter PAT2 (slc36a2).Biochimica et biophysica acta · 2011Article
- Intracellular amino acid sensing and mTORC1-regulated growth: new ways to block an old target?Current opinion in investigational drugs (London, England : 2000) · 2010Review
- Functional expression of two system A glutamine transporter isoforms in rat auditory brainstem neurons.Neuroscience · 2009Article
- Characterization of a blood-meal-responsive proton-dependent amino acid transporter in the disease vector, Aedes aegypti.The Journal of experimental biology · 2009Article
- Human solute carrier SLC6A14 is the beta-alanine carrier.The Journal of physiology · 2008Article
- A glia-neuron alanine/ammonium shuttle is central to energy metabolism in bee retina.The Journal of physiology · 2008Article
- Deletion of v7-3 (SLC6A15) transporter allows assessment of its roles in synaptosomal proline uptake, leucine uptake and behaviors.Brain research · 2007Article
- H+-coupled nutrient, micronutrient and drug transporters in the mammalian small intestine.Experimental physiology · 2007Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Functional characteristics and substrate specificity of the rat proton-coupled amino acid transporter 2 (rat PAT2 (rPAT2)) were determined following expression in Xenopus laevis oocytes using radiolabelled uptake measurements, competition experiments and measurements of substrate-evoked current using the two-electrode voltage-clamp technique. The aim of the investigation was to determine the structural requirements and structural limitations of potential substrates for rPAT2. Amino (and imino) acid transport via rPAT2 was pH-dependent, Na(+)-independent and electrogenic. At extracellular pH 5.5 (in Na(+)-free conditions) proline uptake was saturable (Km 172+/-41 muM), demonstrating that rPAT2 is, relative to PAT1, a high-affinity transporter.PAT2 preferred substrates are L-alpha-amino acids with small aliphatic side chains (e.g. the methyl group in alanine) and 4- or 5-membered heterocyclic amino and imino acids such as 2-azetidine-carboxylate, proline and cycloserine, where both D- and L-enantiomers are transported. The major restrictions on transport are side chain size (the ethyl group of alpha-aminobutyric acid is too large) and backbone length, where the separation of the carboxyl and amino groups by only two CH(2) groups, as in beta-alanine, is enough to reduce transport. Methylation of the amino group is tolerated (e.g. sarcosine) but increasing methylation, as in betaine, decreases transport. A free carboxyl group is preferred as O-methyl esters show either reduced transport (alanine-O-methyl ester) or are excluded. The structural characteristics that determine the substrate specificity of rPAT2 have been identified. This information should prove valuable in the design of selective substrates/inhibitors for PAT1 and PAT2.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.