ArticlePlant physiology2005
An inducible targeted tagging system for localized saturation mutagenesis in Arabidopsis.
Article in Plant physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 31 citations in OpenAlex.
- High-throughput generation of an activation-tagged mutant library for functional genomic analyses in tobacco.Planta · 2015Article
- Transposon based activation tagging in diploid strawberry and monoploid derivatives of potato.Plant cell reports · 2014Article
- MYB10 and MYB72 are required for growth under iron-limiting conditions.PLoS genetics · 2013Article
- The TIE1 transcriptional repressor links TCP transcription factors with TOPLESS/TOPLESS-RELATED corepressors and modulates leaf development in Arabidopsis.The Plant cell · 2013Article
- The rice miniature inverted repeat transposable element mPing is an effective insertional mutagen in soybean.Plant physiology · 2011Article
- Genome rearrangements in maize induced by alternative transposition of reversed ac/ds termini.Genetics · 2011Article
- Perspectives on Systematic Analyses of Gene Function in Arabidopsis thaliana: New Tools, Topics and Trends.Current genomics · 2011Article
- Pea chloroplast DnaJ-J8 and Toc12 are encoded by the same gene and localized in the stroma.Plant physiology · 2010Article
- Phenome analysis in plant species using loss-of-function and gain-of-function mutants.Plant & cell physiology · 2009Review
- Use of the transposable element Ac/Ds in conjunction with Spm/dSpm for gene tagging allows extensive genome coverage with a limited number of starter lines: functional analysis of a four-element system in Arabidopsis thaliana.Molecular genetics and genomics : MGG · 2006Article
- Targeted Ds-tagging strategy generates high allelic diversity at the Arabidopsis HY2 locus.Plant molecular biology · 2006Article
- Article
- Controlled expression of recombinant proteins in Physcomitrella patens by a conditional heat-shock promoter: a tool for plant research and biotechnology.Plant molecular biology · 2005Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a system of inducible insertional mutagenesis based on the Ac-Ds family of transposons for targeted tagging in Arabidopsis (Arabidopsis thaliana). In this system, the Ac and Ds elements are carried within the same T-DNA and a heat shock-inducible transposase fusion is utilized to control the levels of transposase gene expression, generating transpositions that can be subsequently stabilized without requiring crossing or segregation. We have mapped 40 single-copy lines by thermal asymmetric interlaced-PCR, which can be used as potential launch pads for heat shock mutagenesis. Using a starter line selected for detailed analysis, the efficiency of tagging over a 50-kb region in the genome was examined. Hits were obtained in the targeted genes with multiple alleles for most genes, with approximately equal numbers of hits detected in genes on either side of the T-DNA. These results establish the feasibility of our approach for localized saturation mutagenesis in Arabidopsis. This system is very efficient and much less laborious as compared to conventional crossing schemes and may be generally applicable to other plant species for which large-scale T-DNA tagging is not currently feasible.
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