ArticleEukaryotic cell2005
Separation of the Saccharomyces cerevisiae Paf1 complex from RNA polymerase II results in changes in its subnuclear localization.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- PAF1c depletion confers chemoresistance to topoisomerase inhibitors.Cell insight · 2026Article
- A germline PAF1 paralog complex ensures cell type-specific gene expression.Genes & development · 2024Article
- Coordinated Control of rRNA Processing by RNA Polymerase I.Trends in genetics : TIG · 2019Review
- The Paf1 Complex Broadly Impacts the Transcriptome ofGenetics · 2019Article
- Cdc73 suppresses genome instability by mediating telomere homeostasis.PLoS genetics · 2018Article
- Seed dormancy and germination-emerging mechanisms and new hypotheses.Frontiers in plant science · 2014Review
- Characterization of a new CDC73 missense mutation that impairs Parafibromin expression and nucleolar localization.PloS one · 2014Article
- The many roles of the conserved eukaryotic Paf1 complex in regulating transcription, histone modifications, and disease states.Biochimica et biophysica acta · 2013Article
- The PAF1 complex differentially regulates cardiomyocyte specification.Developmental biology · 2011Article
- The RNA polymerase-associated factor 1 complex (Paf1C) directly increases the elongation rate of RNA polymerase I and is required for efficient regulation of rRNA synthesis.The Journal of biological chemistry · 2010Article
- Article
- Genetic identification of factors that modulate ribosomal DNA transcription in Saccharomyces cerevisiae.Genetics · 2009Article
- The Paf1 complex is required for efficient transcription elongation by RNA polymerase I.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Direct interactions between the Paf1 complex and a cleavage and polyadenylation factor are revealed by dissociation of Paf1 from RNA polymerase II.Eukaryotic cell · 2008Article
- Rtf1 is a multifunctional component of the Paf1 complex that regulates gene expression by directing cotranscriptional histone modification.Molecular and cellular biology · 2007Article
- The Spt6 SH2 domain binds Ser2-P RNAPII to direct Iws1-dependent mRNA splicing and export.Genes & development · 2007Article
- Immunohistochemical assessment of parafibromin in mouse and human tissues.Journal of anatomy · 2006Article
- Drosophila Paf1 modulates chromatin structure at actively transcribed genes.Molecular and cellular biology · 2006Article
- Identification of a functional bipartite nuclear localization signal in the tumor suppressor parafibromin.Oncogene · 2005Article
- The Paf1 complex: platform or player in RNA polymerase II transcription?Biochimica et biophysica actaReview
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3 authors at 1 institution in 1 country.
Funding
Abstract
The yeast Paf1 complex (Paf1C), composed of Paf1, Ctr9, Cdc73, Rtf1, and Leo1, associates with RNA polymerase II (Pol II) at promoters and in the actively transcribed portions of mRNA genes. Loss of Paf1 results in severe phenotypes and significantly reduced levels of the other Paf1C components. In contrast, loss of Rtf1 causes relatively subtle phenotypic changes and no reduction in the other Paf1C factors but disrupts the association of these factors with Pol II and chromatin. To elucidate the fate of the Paf1C when dissociated from Pol II, we examined the localization of the Paf1C components in paf1 and rtf1 mutant yeast strains. We found that although the Paf1C factors remain nuclear in paf1 and rtf1 strains, loss of Paf1 or Rtf1 results in a change in the subnuclear distribution of the remaining factors. In wild-type cells, Paf1C components are present in the nucleoplasm but not the nucleolus. In contrast, in both paf1 and rtf1 strains, the remaining factors are found in the nucleolus as well as the nucleoplasm. Loss of Paf1 affects nucleolar function; we observed that expression of MAK21 and RRP12, important for rRNA processing, is reduced concomitant with an increase in rRNA precursors in a paf1 strain. However, these changes are not the result of relocalization of the Paf1C because loss of Rtf1 does not cause similar changes in rRNA processing. Instead, we speculate that the change in localization may reflect a link between the Paf1C and newly synthesized mRNAs as they exit the nucleus.
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