ArticleEukaryotic cell2005
Distinct stress responses of two functional laccases in Cryptococcus neoformans are revealed in the absence of the thiol-specific antioxidant Tsa1.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed, 121 citations in OpenAlex.
- Immune Modulation and Evasion byACS omega · 2026Review
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- Systematic Profiling of Essential Fungal Transcription Factors Uncovers Ezt1 as a Central Pathobiological and Morphogenic Regulator inbioRxiv : the preprint server for biology · 2026Article
- Genetically Encoded Sensors for Monitoring Intracellular Redox Health of the Pathogenic FungusACS sensors · 2025Article
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- Genomic and phenotypic insights into the expanding phylogenetic landscape of the Cryptococcus genus.PLoS genetics · 2025Article
- Genomic and phenotypic insights into the expanding phylogenetic landscape of thebioRxiv : the preprint server for biology · 2025Article
- Glutathione-mediated redox regulation in Cryptococcus neoformans impacts virulence.Nature microbiology · 2024Article
- Measuring Laccase Activity and Melanin Production in Cryptococcus neoformans.Methods in molecular biology (Clifton, N.J.) · 2024Article
- CRISPR-based tools for targeted genetic manipulation in pathogenicMicrobiology spectrum · 2023Article
- The Dynamics ofCells · 2022Article
- Improvement of laccase activity by silencing PacC in Ganoderma lucidum.World journal of microbiology & biotechnology · 2022Article
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- Multicopper Oxidases inJournal of fungi (Basel, Switzerland) · 2020Review
- Benzothiourea Derivatives Target the Secretory Pathway of the Human Fungal PathogenACS infectious diseases · 2020Article
- Manganese (II) removal from aqueous solutions byBiotechnology reports (Amsterdam, Netherlands) · 2020Article
- Contribution of Laccase Expression to Immune Response against Cryptococcus gattii Infection.Infection and immunity · 2020Article
- Host and Pathogen Communication in the Respiratory Tract: Mechanisms and Models of a Complex Signaling Microenvironment.Frontiers in medicine · 2020Review
- Basic principles of the virulence ofVirulence · 2019Review
3 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Laccases are thought to be important to the virulence of many fungal pathogens by producing melanin, a presumed oxygen radical scavenger. A laccase in Cryptococcus neoformans has been shown to synthesize melanin and contributes to the virulence and the survival in macrophages of this fungal pathogen. One C. neoformans laccase gene, LAC1, previously called CNLAC1, has been extensively studied, and we describe a homologous gene, LAC2, that is found 8 kb away from LAC1 in the genome. In this study we report a role for both laccases, in addition to the thiol peroxidase, Tsa1, in oxidative and nitrosative stress resistance mechanisms of C. neoformans. With use of real-time PCR, similar changes in expression of the two laccase genes occur in response to oxidative and nitrosative stresses, but only the regulation of the LAC2 gene during stress is influenced by Tsa1. Both laccases contribute to melanin production using L-dopa as a substrate and are differentially localized in the cell based on green fluorescent protein fusions. A single deletion of either LAC1 or LAC2 alone had no effect on sensitivity to H2O2 or nitric oxide. However, deletion of either LAC1 or LAC2 in combination with a TSA1 deletion resulted in a slight peroxide sensitivity, and a lac2Delta tsa1Delta deletion strain was sensitive to nitric oxide stress. In addition, the deletion of both laccases reduces survival of C. neoformans in primary macrophages. Based on our expression and functional analysis, we propose a novel model for the interaction of these two systems, which are both important for virulence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.