ArticleEukaryotic cell2005
Mcl1p is a polymerase alpha replication accessory factor important for S-phase DNA damage survival.
Article in Eukaryotic cell, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- FANCJ DNA helicase is recruited to the replisome by AND-1 to ensure genome stability.EMBO reports · 2024Article
- Genome-wide analysis of WD40 protein family and functional characterization ofFrontiers in plant science · 2023Article
- The Effect of Dia2 Protein Deficiency on the Cell Cycle, Cell Size, and Recruitment of Ctf4 Protein inMolecules (Basel, Switzerland) · 2021Article
- AND-1 fork protection function prevents fork resection and is essential for proliferation.Nature communications · 2018Article
- Fission Yeast Sirtuin Hst4 Functions in Preserving Genomic Integrity by Regulating Replisome Component Mcl1.Scientific reports · 2018Article
- Dependency of Heterochromatin Domains on Replication Factors.G3 (Bethesda, Md.) · 2018Article
- A genome-wide screen for sporulation-defective mutants in Schizosaccharomyces pombe.G3 (Bethesda, Md.) · 2014Article
- The subunits of the S-phase checkpoint complex Mrc1/Tof1/Csm3: dynamics and interdependence.Cell division · 2014Article
- Continued DNA synthesis in replication checkpoint mutants leads to fork collapse.Molecular and cellular biology · 2012Article
- WDHD1 modulates the post-transcriptional step of the centromeric silencing pathway.Nucleic acids research · 2011Article
- Structure of a DNA polymerase alpha-primase domain that docks on the SV40 helicase and activates the viral primosome.The Journal of biological chemistry · 2010Article
- Physical interactions between Mcm10, DNA, and DNA polymerase alpha.The Journal of biological chemistry · 2009Article
- GINS inactivation phenotypes reveal two pathways for chromatin association of replicative alpha and epsilon DNA polymerases in fission yeast.Molecular biology of the cell · 2009Article
- A DNA polymerase alpha accessory protein, Mcl1, is required for propagation of centromere structures in fission yeast.PloS one · 2008Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Mcl1p is an essential fission yeast chromatin-binding protein that belongs to a family of highly conserved eukaryotic proteins important for sister chromatid cohesion. The essential function is believed to result from its role as a Pol1p (polymerase alpha) accessory protein, a conclusion based primarily on analogy to Ctf4p's interaction with Pol1p. In this study, we show that Mcl1p also binds to Pol1p with high affinity for the N terminus of Pol1p during S phase and DNA damage. Characterization of an inducible allele of mcl1+, (nmt41)mcl1-MH, shows that altered expression levels of Mcl1p lead to sensitivity to DNA-damaging agents and synthetic lethality with the replication checkpoint mutations rad3Delta, rqh1Delta, and hsk1-1312. Further, we find that the overexpression of the S-phase checkpoint kinase, Cds1, or the loss of Hsk1 kinase activity can disrupt Mcl1p's interaction with chromatin and Pol1p during replication arrest with hydroxyurea. We take these data to mean that Mcl1p is a dynamic component of the polymerase alpha complex during replication and is important for the replication stress response in fission yeast.
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