ArticleClinical and diagnostic laboratory immunology2005
Efficacy of several serological tests and antigens for diagnosis of bovine brucellosis in the presence of false-positive serological results due to Yersinia enterocolitica O:9.
Article in Clinical and diagnostic laboratory immunology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
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Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it, 149 citations in OpenAlex.
- Epidemiology of Brucellosis Among Deer in China From 1978 to 2025: A Systematic Review and Meta-Analysis.Transboundary and emerging diseases · 2026Pooled it
- Serological evaluation of brucellosis in nomadic small ruminants in Southeastern Türkiye.BMC veterinary research · 2026Article
- Occurrence of viral and bacterial pathogens in South American camelids in representative German flocks.Frontiers in veterinary science · 2026Article
- Ghostbuster-A Western Blot-Based Panel Method to Resolve False-Positive Brucellosis Serology Test Results.Microorganisms · 2025Article
- Exploring potential cytokine profiles as diagnostic biomarkers for brucellosis in Mediterranean Buffaloes.Frontiers in veterinary science · 2025Article
- Seroprevalence and Risk Factors Associated With Brucellosis in Goats in Nyagatare District, Rwanda.Veterinary medicine international · 2025Article
- Disseminated Yersinia enterocolitica infection associated to Sweet's syndrome.BMC infectious diseases · 2024Article
- Seroprevalence and molecular detection of brucellosis among Pakistani women with spontaneous abortion.Frontiers in public health · 2024Article
- Seroprevalence and spatial distribution of livestock brucellosis using three serological tests in Kajiado County, Kenya.Open veterinary journal · 2023Article
- Article
- False Positives in Brucellosis Serology: Wrong Bait and Wrong Pond?Tropical medicine and infectious disease · 2023Review
- Human brucellosis in Baringo County, Kenya: Evaluating the diagnostic kits used and identifying infecting Brucella species.PloS one · 2023Article
- Development of a Multiplex Bead Assay to Detect Serological Responses toMicroorganisms · 2022Article
- A Brucella melitensis H38ΔwbkF rough mutant protects against Brucella ovis in rams.Veterinary research · 2022Article
- Evaluation of Hematological, Oxidative Stress, and Antioxidant Profile in Cattle Infected with Brucellosis in Southern Punjab, Pakistan.BioMed research international · 2022Article
- Bovine Brucellosis: Epidemiology, Public Health Implications, and Status of Brucellosis in Ethiopia.Veterinary medicine (Auckland, N.Z.) · 2022Review
- Article
- Proteomic and Antibody Profiles Reveal Antigenic Composition and Signatures of Bacterial Ghost Vaccine ofFrontiers in immunology · 2022Article
- Comparative evaluation of the immunodominant proteins ofVeterinary world · 2021Article
- Brucella abortus S19 GFP-tagged vaccine allows the serological identification of vaccinated cattle.PloS one · 2021Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Yersinia enterocolitica O:9 bears a smooth lipopolysaccharide (S-LPS) of Brucella sp. O-chain A+C/Y epitopic structure and is a cause of false-positive serological reactions (FPSR) in standard tests for cattle brucellosis. Brucella S-LPS, cross-reacting S-LPSs representing several O-chain epitope combinations, Brucella core lipid A epitopes (rough LPS), Brucella abortus S-LPS-derived polysaccharide, native hapten polysaccharide, rough LPS group 3 outer membrane protein complexes, recombinant BP26, and cytosolic proteins were tested in enzyme-linked immunosorbent assays (ELISA) and precipitation tests to detect cattle brucellosis (sensitivity) and to differentiate it from FPSR (specificity). No single serological test and antigen combination showed 100% sensitivity and specificity simultaneously. Immunoprecipitation tests with native hapten polysaccharide, counterimmunoelectrophoresis with cytosolic proteins, and a chaotropic ELISA with Brucella S-LPS were 100% specific but less sensitive than the Rose Bengal test, complement fixation, and indirect ELISA with Brucella S-LPSs and native hapten or S-LPS-derived polysaccharides. A competitive ELISA with Brucella S-LPS and M84 C/Y-specific monoclonal antibody was not 100% specific and was less sensitive than other tests. ELISA with Brucella suis bv. 2 S-LPS (deficient in C epitopes), Escherichia hermannii S-LPSs [lacking the contiguous alpha-(1-2)-linked perosamine residues characteristic of Y. enterocolitica S-LPS], BP26 recombinant protein, and Brucella cytosolic fractions did not provide adequate sensitivity/specificity ratios. Although no serological test and antigen combination fully resolved the diagnosis of bovine brucellosis in the presence of FPSR, some are simple and practical alternatives to the brucellin skin test currently recommended for differential diagnosis.
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