ArticleNucleic acids research2005
A precise DNA bend angle is essential for the function of the phage phi29 transcriptional regulator.
Article in Nucleic acids research, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- Analysis of single, cisplatin-induced DNA bends by atomic force microscopy and simulations.Journal of molecular recognition : JMR · 2018Article
- PT-ACRAMTU, a platinum-acridine anticancer agent, lengthens and aggregates, but does not stiffen or soften DNA.Cell biochemistry and biophysics · 2013Article
- Functional specificity of a protein-DNA complex mediated by two arginines bound to the minor groove.Journal of bacteriology · 2012Article
- Molecular interactions and protein-induced DNA hairpin in the transcriptional control of bacteriophage ø29 DNA.International journal of molecular sciences · 2010Review
- Tuning the relative affinities for activating and repressing operators of a temporally regulated restriction-modification system.Nucleic acids research · 2009Article
- DNA sequence-specific recognition by a transcriptional regulator requires indirect readout of A-tracts.Nucleic acids research · 2007Article
- Homologies and divergences in the transcription regulatory system of two related Bacillus subtilis phages.Journal of bacteriology · 2005Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Bacteriophage phi29 protein p4 is essential for the regulation of the switch from early to late phage transcription. The protein binds to two regions of the phage genome located between the regulated promoters. Each region contains two inverted repeats separated by 1 bp. We used circular permutation assays to study the topology of the DNA upon binding of the protein and found that p4 induced the same extent of bending independent of the topology of the binding region. In addition, the results revealed that the p4-induced bending is not dependent on the affinity to the binding site but is intrinsic to p4 binding. Independent binding sites were identified through the characterization of the minimal sequence required for p4 binding. The protein has different affinity for each of its binding sites, with those overlapping the A2c and A2b promoter cores (sites 1 and 3), having the highest affinity. The functionality of the p4 binding sites and the contribution of p4-mediated promoter restructuring in transcription regulation is discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.