ArticleWorld journal of gastroenterology2005
Detection of hepatitis B virus DNA by real-time PCR using TaqMan-MGB probe technology.
Article in World journal of gastroenterology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Article
- Nanoparticle-Based Lateral Flow Biosensors Integrated With Loop-Mediated Isothermal Amplification for the Rapid and Visual Diagnosis of Hepatitis B Virus in Clinical Application.Frontiers in bioengineering and biotechnology · 2021Article
- Multiple Cross Displacement Amplification Linked with Nanoparticles-Based Lateral Flow Biosensor in Screening of Hepatitis B Virus in Clinical Application.Infection and drug resistance · 2021Article
- An in-house real-time polymerase chain reaction: standardisation and comparison with the Cobas Amplicor HBV monitor and Cobas AmpliPrep/Cobas TaqMan HBV tests for the quantification of hepatitis B virus DNA.Memorias do Instituto Oswaldo Cruz · 2016Article
- The effect of acute dose charge particle radiation on expression of DNA repair genes in mice.Molecular and cellular biochemistry · 2011Article
- Sensitive assay for quantification of hepatitis B virus mutants by use of a minor groove binder probe and peptide nucleic acids.Journal of clinical microbiology · 2010Article
- Rapid detection of H5 avian influenza virus by TaqMan-MGB real-time RT-PCR.Letters in applied microbiology · 2008Article
- Association of cell-free plasma DNA with hospital mortality and organ dysfunction in intensive care unit patients.Intensive care medicine · 2007Article
- COBAS AmpliPrep-COBAS TaqMan hepatitis B virus (HBV) test: a novel automated real-time PCR assay for quantification of HBV DNA in plasma.Journal of clinical microbiology · 2007Article
- Rapid quantification of hepatitis B virus DNA by real-time PCR using efficient TaqMan probe and extraction of virus DNA.World journal of gastroenterology · 2006Article
- Large scale real-time PCR validation on gene expression measurements from two commercial long-oligonucleotide microarrays.BMC genomics · 2006Article
- Molecular diagnosis of severe acute respiratory syndrome.Methods in molecular biology (Clifton, N.J.) · 2006Article
- Automated extraction of viral-pathogen RNA and DNA for high-throughput quantitative real-time PCR.Journal of clinical microbiology · 2005Article
- Detection and monitoring of virus infections by real-time PCR.Molecular aspects of medicineReview
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimTo develop a real-time PCR for detecting hepatitis B virus (HBV) DNA based on TaqMan technology using a new MGB probe.
methodsPlasmid containing the sequence of X gene (1414-1744 nt) was constructed as HBV-DNA standard for quantitative analysis. A TaqMan-MGB probe between primers for amplification was designed to detect PCR products. The interested sequence contained in the plasmid and in clinical specimens was quantitatively measured.
resultsThe detection limit of the assay for HBV DNA was 1 genome equivalent per reaction. A linear standard curve was obtained between 10(0) and 10(9) DNA copies/reaction (r>0.990). None of the negative control samples showed false-positive reactions in duplicate. HBV DNA was detected in 100% (50/50) of HBV patients with HbeAg, and in 72.0% (36/50) with HBsAg, HBeAb and HBcAb. The coefficient of variation for both intra- and inter-experimental variability demonstrated high reproducibility and accuracy.
conclusionReal-time PCR based on TaqMan-MGB probe technology is an excellent method for detection of HBV DNA.
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