ArticleBone marrow transplantation2005
A prospective study of real-time panfungal PCR for the early diagnosis of invasive fungal infection in haemato-oncology patients.
Article in Bone marrow transplantation, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 2 of them syntheses that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 2 syntheses or guidelines pooled it, 100 citations in OpenAlex.
- Polymerase chain reaction blood tests for the diagnosis of invasive aspergillosis in immunocompromised people.The Cochrane database of systematic reviews · 2019Pooled it
- PCR in diagnosis of invasive aspergillosis: a meta-analysis of diagnostic performance.Journal of clinical microbiology · 2014Pooled it
- New strategy for rapid diagnosis and characterization of fungal infections: the example of corneal scrapings.PloS one · 2012Trial
- Randomized PCR-based therapy and risk factors for invasive fungal infection following reduced-intensity conditioning and hematopoietic SCT.Bone marrow transplantation · 2010Trial
- Speciation analysis of fungi by liquid atmospheric pressure MALDI mass spectrometry.Analytical and bioanalytical chemistry · 2025Article
- Investigation of adhesion status of Candida species to the surface of resin materials produced at different angles with additive manufacturing.BMC oral health · 2024Article
- Utility of Panfungal PCR in the diagnosis of invasive fungal infections in febrile neutropenia.Journal of family medicine and primary care · 2021Article
- Molecular Diagnosis of Yeast Infections.Current fungal infection reports · 2021Review
- Clinical evaluation of an in-house panfungal real-time PCR assay for the detection of fungal pathogens.Infection · 2020Article
- High incidence of invasive fungal infection during acute myeloid leukemia treatment in a resource-limited country: clinical risk factors and treatment outcomes.Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer · 2019Article
- Speciation of fungi using real time PCR with molecular beacons: Can we solve the enigma of diagnosis of invasive fungal disease?Medical journal, Armed Forces India · 2019Article
- New Panfungal Real-Time PCR Assay for Diagnosis of Invasive Fungal Infections.Journal of clinical microbiology · 2016Article
- Similar efficacy of broad-range ITS PCR and conventional fungal culture for diagnosing fungal infections in non-immunocompromised patients.BMC microbiology · 2016Article
- Application of Culture-Independent Rapid Diagnostic Tests in the Management of Invasive Candidiasis and Cryptococcosis.Journal of fungi (Basel, Switzerland) · 2015Review
- Detection of tropical fungi in formalin-fixed, paraffin-embedded tissue: still an indication for microscopy in times of sequence-based diagnosis?BioMed research international · 2015Article
- Assessing responses to treatment of opportunistic mycoses and salvage strategies.Current infectious disease reports · 2011Article
- Diagnosis of invasive fungal infections by a real-time panfungal PCR assay in immunocompromised pediatric patients.Leukemia · 2010Article
- Detection of Aspergillus fumigatus in a rat model of invasive pulmonary aspergillosis by real-time nucleic acid sequence-based amplification.Journal of clinical microbiology · 2010Article
- PCR-based diagnosis of human fungal infections.Expert review of anti-infective therapy · 2009Review
- Species-specific identification of a wide range of clinically relevant fungal pathogens by use of Luminex xMAP technology.Journal of clinical microbiology · 2009Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A blinded prospective study was performed to determine whether screening of whole blood using a real-time, panfungal polymerase chain reaction (PCR) technique could predict the development of invasive fungal infection (IFI) in immunocompromised haemato-oncology patients. In all, 78 patients (125 treatment episodes) were screened twice weekly by real-time panfungal PCR using LightCyclertrade mark technology. IFI was documented in 19 treatment episodes (five proven, three probable and 11 possible), and in 12, PCR was sequentially positive. PCR positivity occurred in: 4/5 proven; 2/3 probable; 6/11 possible; and 29/106 with no IFI. In 8/12 with IFI and sequentially positive PCR results, PCR positivity occurred before (median 19.5 days) and in 4/12 (median 10.5 days) after the initiation of empirical antifungal therapy. Based on sequential positive results for proven/probable IFI sensitivity, specificity, positive predictive value and negative predictive value were 75, 70, 15 and 98%, respectively. Real-time panfungal PCR is a sensitive tool for the early diagnosis of IFI in immunocompromised haemato-oncology patients. It may be most useful as a screening method in high-risk patients, either to direct early pre-emptive antifungal therapy or to determine when empirical antifungal therapy can be withheld in patients with antibiotic--resistant neutropenic fever. However, these strategies require further assessment in comparative clinical trials.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.