ArticleApplied and environmental microbiology2005
High-level overproduction of His-tagged Tth DNA polymerase in Thermus thermophilus.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- The Structural Proteins of Thermophilic Bacteriophage P23-77: Expression and Characterization.International journal of molecular sciences · 2025Article
- Design, Co-Expression, and Evaluation for Assembly of the Structural Proteins from Thermophilic Bacteriophage ΦIN93.International journal of molecular sciences · 2025Article
- Exploitation of Biodiversity in Bioeconomy: Examples, Opportunities, and Challenges.Advances in biochemical engineering/biotechnology · 2025Review
- Extremophiles in a changing world.Extremophiles : life under extreme conditions · 2024Review
- A temperature dependent pilin promoter for production of thermostable enzymes in Thermus thermophilus.Microbial cell factories · 2023Article
- Development of a new gene expression vector for Thermus thermophilus using a silica-inducible promoter.Microbial cell factories · 2020Article
- Genome-wide systematic identification of methyltransferase recognition and modification patterns.Nature communications · 2019Article
- Galactomannan degradation by thermophilic enzymes: a hot topic for biotechnological applications.World journal of microbiology & biotechnology · 2019Review
- Physiological, metabolic and biotechnological features of extremely thermophilic microorganisms.Wiley interdisciplinary reviews. Systems biology and medicine · 2017Review
- Thermus thermophilus as source of thermozymes for biotechnological applications: homologous expression and biochemical characterization of an α-galactosidase.Microbial cell factories · 2017Article
- Extremely thermophilic microorganisms as metabolic engineering platforms for production of fuels and industrial chemicals.Frontiers in microbiology · 2015Review
- Phosphoproteomic analysis reveals the effects of PilF phosphorylation on type IV pilus and biofilm formation in Thermus thermophilus HB27.Molecular & cellular proteomics : MCP · 2013Article
- Polysaccharide-degrading thermophiles generated by heterologous gene expression in Geobacillus kaustophilus HTA426.Applied and environmental microbiology · 2013Article
- Identification of a replication initiation protein of the pVV8 plasmid from Thermus thermophilus HB8.Extremophiles : life under extreme conditions · 2013Article
- Characterization of the PIB-Type ATPases present in Thermus thermophilus.Journal of bacteriology · 2012Article
- Importance of the latex-clearing protein (Lcp) for poly(cis-1,4-isoprene) rubber cleavage in Streptomyces sp. K30.MicrobiologyOpen · 2012Article
- Thermus thermophilus as biological model.Extremophiles : life under extreme conditions · 2009Review
- Omp85(Tt) from Thermus thermophilus HB27: an ancestral type of the Omp85 protein family.Journal of bacteriology · 2008Article
- Characterization of three putative Lon proteases of Thermus thermophilus HB27 and use of their defective mutants as hosts for production of heterologous proteins.Extremophiles : life under extreme conditions · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new plasmid for the overexpression of His-tagged thermozymes in Thermus thermophilus was developed. With this plasmid, soluble and active histidine-tagged DNA polymerase from T. thermophilus was overproduced in larger amounts in the thermophile than in Escherichia coli. The protein purified from the thermophile was active in PCR.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.