ArticleApplied and environmental microbiology2005
Gene structure and transcriptional organization of the dnaK operon of Bifidobacterium breve UCC 2003 and application of the operon in bifidobacterial tracing.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 73 citations in OpenAlex.
- Bioremoval of lead (pb) salts from synbiotic milk by lactic acid bacteria.Scientific reports · 2025Article
- Review
- Stress Response in Bifidobacteria.Microbiology and molecular biology reviews : MMBR · 2022Review
- Genome-Wide Assessment of Stress-Associated Genes in Bifidobacteria.Applied and environmental microbiology · 2022Article
- Molecular analysis of the replication functions of the bifidobacterial conjugative megaplasmid pMP7017.Microbial biotechnology · 2021Article
- Decoding the Genomic Variability among Members of theMicroorganisms · 2020Article
- A dual-chain assembly pathway generates the high structural diversity of cell-wall polysaccharides inThe Journal of biological chemistry · 2019Article
- Bifidobacterium breve UCC2003 Employs Multiple Transcriptional Regulators To Control Metabolism of Particular Human Milk Oligosaccharides.Applied and environmental microbiology · 2018Article
- Article
- Heat Acclimation of Bifidobacterium longum and Proteomic Changes Behind It.Probiotics and antimicrobial proteins · 2017Article
- Glycosulfatase-Encoding Gene Cluster in Bifidobacterium breve UCC2003.Applied and environmental microbiology · 2016Article
- Transcription of two adjacent carbohydrate utilization gene clusters in Bifidobacterium breve UCC2003 is controlled by LacI- and repressor open reading frame kinase (ROK)-type regulators.Applied and environmental microbiology · 2014Article
- Quantification of human fecal bifidobacterium species by use of quantitative real-time PCR analysis targeting the groEL gene.Applied and environmental microbiology · 2012Article
- Global genome transcription profiling of Bifidobacterium bifidum PRL2010 under in vitro conditions and identification of reference genes for quantitative real-time PCR.Applied and environmental microbiology · 2011Article
- Enhancing the stress responses of probiotics for a lifestyle from gut to product and back again.Microbial cell factories · 2011Review
- Genomic insights into bifidobacteria.Microbiology and molecular biology reviews : MMBR · 2010Review
- HspR mutations are naturally selected in Bifidobacterium longum when successive heat shock treatments are applied.Journal of bacteriology · 2010Article
- An interactive regulatory network controls stress response in Bifidobacterium breve UCC2003.Journal of bacteriology · 2009Article
- Exploring the diversity of the bifidobacterial population in the human intestinal tract.Applied and environmental microbiology · 2009Article
- Detection of Bifidobacterium animalis subsp. lactis (Bb12) in the intestine after feeding of sows and their piglets.Applied and environmental microbiology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The incorporation and delivery of bifidobacterial strains as probiotic components in many food preparations expose these microorganisms to a multitude of environmental insults, including heat and osmotic stresses. We characterized the dnaK gene region of Bifidobacterium breve UCC 2003. Sequence analysis of the dnaK locus revealed four genes with the organization dnaK-grpE-dnaJ-ORF1, whose deduced protein products display significant similarity to corresponding chaperones found in other bacteria. Northern hybridization and real-time LightCycler PCR analysis revealed that the transcription of the dnaK operon was strongly induced by osmotic shock but was not induced significantly by heat stress. A 4.4-kb polycistronic mRNA, which represented the transcript of the complete dnaK gene region, was detected. Many other small transcripts, which were assumed to have resulted from intensive processing or degradation of this polycistronic mRNA, were identified. The transcription start site of the dnaK operon was determined by primer extension. Phylogenetic analysis of the available bifidobacterial grpE and dnaK genes suggested that the evolutionary development of these genes has been similar. The phylogeny derived from the various bifidobacterial grpE and dnaK sequences is consistent with that derived from 16S rRNA. The use of these genes in bifidobacterial species as an alternative or complement to the 16S rRNA gene marker provides sequence signatures that allow a high level of discrimination between closely related species of this genus.
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Registered trials
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