ArticleApplied and environmental microbiology2005
Cloning of Beauveria bassiana chitinase gene Bbchit1 and its application to improve fungal strain virulence.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers.
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85 citing papers in PubMed, 349 citations in OpenAlex.
- A mycovirus shaped insect-pathogenic and non-pathogenic phenotypes in a fungal biocontrol agent.Virulence · 2026Article
- The role of fungal chitinases in the tripartite interactions among insects, plants and entomopathogenic fungi.Pest management science · 2026Review
- Integrated genomic and phenotypic analysis of an endophytic bacterium reveals biocontrol and plant growth-promoting mechanisms.iScience · 2026Article
- Gene presence-absence and evolutionary signals of positive selection associated with virulence divergence inMicrobial genomics · 2026Article
- Multi-locus phylogeny and cuticle-degrading enzyme activities of entomopathogenic fungi from forest ecosystems in Northern Thailand.World journal of microbiology & biotechnology · 2026Article
- Potential of endophytic Beauveria bassiana against Coraebus (Coleoptera: Buprestidae) oak borers.Pest management science · 2026Article
- Genetic engineering of Beauveria bassiana for enhancing siderophore production and its virulence against the insect hosts.Biotechnology letters · 2026Article
- The Factors Involved in Plant-Insect-Microbe Interactions Expanded: Genome Analysis and Description of Frigoribacterium adelgis sp. nov.Environmental microbiology reports · 2026Article
- The chitinolytic enzymes from the entomopathogenic fungusFrontiers in fungal biology · 2026Review
- Biochemical analyses of a new GH18 chitinase fromApplied and environmental microbiology · 2025Article
- Host range and virulence of Metarhizium pingshaense against Chilo species and expression of fungal virulence genes in Conogethes punctiferalis.Scientific reports · 2025Article
- Genome Sequence, Comparative Genome Analysis, and Expression Profiling of the Chitinase GH18 Gene Family inInternational journal of molecular sciences · 2025Article
- Article
- Beauveria bassiana transcriptomics reveal virulence-associated shifts during insect lipid assimilation.Applied microbiology and biotechnology · 2024Article
- Exploring secretory signal sequences useful in excreting recombinant proteins in Beauveria bassiana as biocontrol fungus.Archives of microbiology · 2024Article
- Engineered fungus containing a caterpillar gene kills insects rapidly by disrupting their ecto- and endo-microbiomes.Communications biology · 2024Article
- Metabolomic profiling of virulent and non-virulent Beauveria bassiana strains: insights into the pathogenicity of Tetranychus truncatus.Archives of microbiology · 2024Article
- Comprehensive Insights into the Remarkable Function and Regulatory Mechanism of FluG during Asexual Development inInternational journal of molecular sciences · 2024Article
- From phyllosphere to insect cuticles: silkworms gather antifungal bacteria from mulberry leaves to battle fungal parasite attacks.Microbiome · 2024Article
- Computational identification and characterization of chitinase 1 and chitinase 2 from neotropical isolates ofFrontiers in bioinformatics · 2024Article
25 more citing papers are in PubMed but not listed here.
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10 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Entomopathogenic fungi can produce a series of chitinases, some of which act synergistically with proteases to degrade insect cuticle. However, chitinase involvement in insect fungus pathogenesis has not been fully characterized. In this paper, an endochitinase, Bbchit1, was purified to homogeneity from liquid cultures of Beauveria bassiana grown in a medium containing colloidal chitin. Bbchit1 had a molecular mass of about 33 kDa and pI of 5.4. Based on the N-terminal amino acid sequence, the chitinase gene, Bbchit1, and its upstream regulatory sequence were cloned. Bbchit1 was intronless, and there was a single copy in B. bassiana. Its regulatory sequence contained putative CreA/Crel carbon catabolic repressor binding domains, which was consistent with glucose suppression of Bbchit1. At the amino acid level, Bbchit1 showed significant similarity to a Streptomyces avermitilis putative endochitinase, a Streptomyces coelicolor putative chitinase, and Trichoderma harzianum endochitinase Chit36Y. However, Bbchit1 had very low levels of identity to other chitinase genes previously isolated from entomopathogenic fungi, indicating that Bbchit1 was a novel chitinase gene from an insect-pathogenic fungus. A gpd-Bbchit1 construct, in which Bbchit1 was driven by the Aspergiullus nidulans constitutive promoter, was transformed into the genome of B. bassiana, and three transformants that overproduced Bbchit1 were obtained. Insect bioassays revealed that overproduction of Bbchit1 enhanced the virulence of B. bassiana for aphids, as indicated by significantly lower 50% lethal concentrations and 50% lethal times of the transformants compared to the values for the wild-type strain.
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