ArticleApplied and environmental microbiology2005
Isolation of auxotrophic mutants of diploid industrial yeast strains after UV mutagenesis.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- UV-B-induced DNA repair mechanisms and their effects on mutagenesis and culturability inmSystems · 2026Article
- A novel yeast extract from a multi-stress-resistant Saccharomyces cerevisiae confers potent antioxidant and UV-protective activities to skin cells.BMC microbiology · 2026Article
- Insertion orientation within the cassette affects gene-targeting success during ends-out recombination in the yeast Saccharomyces cerevisiae.Current genetics · 2022Article
- Truth in wine yeast.Microbial biotechnology · 2022Review
- Methionine and Glycine Stabilize Mitochondrial Activity in Sake Yeast During Ethanol Fermentation.Food technology and biotechnology · 2019Article
- Amino acid substitution scoring matrices specific to intrinsically disordered regions in proteins.Scientific reports · 2019Article
- Increased Acetate Ester Production of Polyploid Industrial Brewer's Yeast Strains via Precise and Seamless "Self-cloning" Integration Strategy.Iranian journal of biotechnology · 2019Article
- Natural Variation inG3 (Bethesda, Md.) · 2018Article
- Selection of PofFrontiers in microbiology · 2018Article
- Mutation accumulation under UV radiation in Escherichia coli.Scientific reports · 2017Article
- Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice.Journal of visualized experiments : JoVE · 2016Article
- The metabolic background is a global player in Saccharomyces gene expression epistasis.Nature microbiology · 2016Article
- Article
- Study of Saccharomyces cerevisiae wine strains for breeding through fermentation efficiency and tetrad analysis.Current microbiology · 2015Article
- Construction of a quadruple auxotrophic mutant of an industrial polyploid saccharomyces cerevisiae strain by using RNA-guided Cas9 nuclease.Applied and environmental microbiology · 2014Article
- Improving industrial yeast strains: exploiting natural and artificial diversity.FEMS microbiology reviews · 2014Review
- Enhancement of ethanol fermentation in Saccharomyces cerevisiae sake yeast by disrupting mitophagy function.Applied and environmental microbiology · 2014Article
- Functional heterologous protein expression by genetically engineered probiotic yeast Saccharomyces boulardii.PloS one · 2014Article
- Generation of a Uracil Auxotroph Strain of the Probiotic Yeast Saccharomyces boulardii as a Host for the Recombinant Protein Production.Avicenna journal of medical biotechnology · 2013Article
- Genetic characterization and construction of an auxotrophic strain of Saccharomyces cerevisiae JP1, a Brazilian industrial yeast strain for bioethanol production.Journal of industrial microbiology & biotechnology · 2012Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Auxotrophic mutants of the yeast Saccharomyces cerevisiae are usually isolated in haploid strains because the isolation of recessive mutations in diploids is thought to be difficult due to the presence of two sets of genes. We show here that auxotrophic mutants of diploid industrial sake yeast strains were routinely obtained by a standard mutant selection procedure following UV mutagenesis. We isolated His(-), Met(-), Lys(-), Trp(-), Leu(-), Arg(-), and Ura(-) auxotrophic mutants of five sake strains, Kyokai no. 7, no. 9, no. 10, no. 701, and no. 901, by screening only 1,700 to 3,400 colonies from each treated strain. Wild-type alleles were cloned and used as markers for transformation. With HIS3 as a selectable marker, the yeast TDH3 overexpression promoter was inserted upstream of ATF1, encoding alcohol acetyltransferase, by one-step gene replacement in a his3 mutant of Kyokai no. 7. The resulting strain contained exclusively yeast DNA, making it acceptable for commercial use, and produced a larger amount of isoamyl acetate, a banana-like flavor. We argue that the generally recognized difficulty of isolating auxotrophic mutants of diploid industrial yeast strains is misleading and that genetic techniques used for haploid laboratory strains are applicable for this purpose.
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