ArticleApplied and environmental microbiology2005
Diversity of green-like and red-like ribulose-1,5-bisphosphate carboxylase/oxygenase large-subunit genes (cbbL) in differently managed agricultural soils.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed.
- Soil pH and Nitrogen Content Drive the Succession of RubisCO-Harboring Microbial Communities AcrossBiology · 2026Article
- Straw application promotes soil carbon storage by affecting aggregate-associated bacterial community structure and RuBisCO activity: a 35-year field experiment.Microbiology spectrum · 2025Article
- Community Composition and Diversity of β-Glucosidase Genes in Soils by Amplicon Sequence Variant Analysis.Genes · 2025Article
- A potential COJournal of advanced research · 2025Article
- Differential diversity and structure of autotrophs in agricultural soils of Qinghai Province.Microbiology spectrum · 2025Article
- Regulatory mechanisms of simulated precipitation on cbbL carbon-fixing microbial communities in the alpine source wetland.Frontiers in microbiology · 2025Article
- Soil microbial COFrontiers in microbiology · 2024Article
- Dynamic Response of the cbbL Carbon Sequestration Microbial Community to Wetland Type in Qinghai Lake.Biology · 2023Article
- Role of Agricultural Management in the Provision of Ecosystem Services in Warm Climate Vineyards: Functional Prediction of Genes Involved in Nutrient Cycling and Carbon Sequestration.Plants (Basel, Switzerland) · 2023Article
- Theoretical design and process control of neonicotinoids insecticides suitable for synergistic degradation with the rubisco enzyme from rhizobia and carbon-fixing bacteria in soil.Environmental science and pollution research international · 2022Article
- Effects of Nitrogen Addition on Soil Carbon-Fixing Microbial Diversity on Different Slopes in a Degraded Alpine Meadow.Frontiers in plant science · 2022Article
- The Impact of Fertilizer Amendments on Soil Autotrophic Bacteria and Carbon Emissions in Maize Field on the Semiarid Loess Plateau.Frontiers in microbiology · 2021Article
- A novel real-world ecotoxicological dataset of pelagic microbial community responses to wastewater.Scientific data · 2020Article
- Specific detection of form IA RubisCO genes in chemoautotrophic bacteria.Journal of basic microbiology · 2018Article
- A short history of RubisCO: the rise and fall (?) of Nature's predominant COCurrent opinion in biotechnology · 2018Review
- Soil Carbon-Fixation Rates and Associated Bacterial Diversity and Abundance in Three Natural Ecosystems.Microbial ecology · 2017Article
- Ammonia-Oligotrophic and Diazotrophic Heavy Metal-Resistant Serratia liquefaciens Strains from Pioneer Plants and Mine Tailings.Microbial ecology · 2016Article
- Ecological restoration alters microbial communities in mine tailings profiles.Scientific reports · 2016Article
- Effect of simulated tillage on microbial autotrophic CO2 fixation in paddy and upland soils.Scientific reports · 2016Article
- Analysis of cbbL, nifH, and pufLM in Soils from the Sør Rondane Mountains, Antarctica, Reveals a Large Diversity of Autotrophic and Phototrophic Bacteria.Microbial ecology · 2016Article
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3 authors.
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Abstract
A PCR-based approach was developed to detect ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) form I large-subunit genes (cbbL) as a functional marker of autotrophic bacteria that fix carbon dioxide via the Calvin-Benson-Bassham cycle. We constructed two different primer sets, targeting the green-like and red-like phylogenetic groups of cbbL genes. The diversity of these cbbL genes was analyzed by the use of three differently managed agricultural soils from a long-term field experiment. cbbL gene fragments were amplified from extracted soil DNAs, and PCR products were cloned and screened by restriction fragment length polymorphism analysis. Selected unique cbbL clones were sequenced and analyzed phylogenetically. The green-like cbbL sequences revealed a very low level of diversity, being closely related to the cbbL genes of Nitrobacter winogradskyi and Nitrobacter vulgaris. In contrast, the red-like cbbL gene libraries revealed a high level of diversity in the two fertilized soils and less diversity in unfertilized soil. The majority of environmental red-like cbbL genes were only distantly related to already known cbbL sequences and even formed separate clusters. In order to extend the database of available red-like cbbL sequences, we amplified cbbL sequences from bacterial type culture strains and from bacterial isolates obtained from the investigated soils. Bacterial isolates harboring the cbbL gene were analyzed phylogenetically on the basis of their 16S rRNA gene sequences. These analyses revealed that bacterial genera such as Bacillus, Streptomyces, and Arthrobacter harbor red-like cbbL genes which fall into the cbbL gene clusters retrieved from the investigated soils.
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