ArticleApplied and environmental microbiology2005
Identification and partial characterization of the nonribosomal peptide synthetase gene responsible for cereulide production in emetic Bacillus cereus.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
88 citing papers in PubMed, 283 citations in OpenAlex.
- Bacterial extracellular vesicles as recyclable nutrient reservoirs.Nature communications · 2026Article
- Biofilm formation, cell hydrophobicity, cytotoxic potential, genetic diversity, resistance to antimicrobials, and toxigenic profile properties amongFood science and biotechnology · 2026Article
- The major toxins of Bacillus cereus - Current knowledge on distribution, regulation and mechanism of action.EXCLI journal · 2026Review
- Genomic and phenotypic diversities among Bacillus cereus isolates from cosmetics.BMC microbiology · 2025Article
- Article
- A taste of one's own medicine:Frontiers in immunology · 2025Article
- Assessment of the risk associated withFrontiers in microbiology · 2025Article
- Genetic Profile and Toxigenic Potential ofFoods (Basel, Switzerland) · 2024Article
- The megaplasmid pCER270 ofApplied and environmental microbiology · 2024Article
- Article
- Genetic Characterization, Antibiotic Resistance, and Virulence Genes Profiling ofAntibiotics (Basel, Switzerland) · 2024Article
- A Pilot Study for the Characterization ofMicroorganisms · 2024Article
- Article
- Meat and meat products as potential sources of emerging MDR Bacillus cereus: groEL gene sequencing, toxigenic and antimicrobial resistance.BMC microbiology · 2024Article
- A poisonous cocktail: interplay of cereulide toxin and its structural isomers in emeticFrontiers in cellular and infection microbiology · 2024Article
- Characterization of the Toxigenic Potential ofFoods (Basel, Switzerland) · 2023Article
- Article
- High Genetic Diversity and Virulence Potential inFoods (Basel, Switzerland) · 2023Article
- The Genetic and Phenotypic Diversity ofMarine drugs · 2023Article
- Intraspecific Diversity and Pathogenicity ofToxins · 2023Article
28 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cereulide, a depsipeptide structurally related to valinomycin, is responsible for the emetic type of gastrointestinal disease caused by Bacillus cereus. Due to its chemical structure, (D-O-Leu-D-Ala-L-O-Val-L-Val)(3), cereulide might be synthesized nonribosomally. Therefore, degenerate PCR primers targeted to conserved sequence motifs of known nonribosomal peptide synthetase (NRPS) genes were used to amplify gene fragments from a cereulide-producing B. cereus strain. Sequence analysis of one of the amplicons revealed a DNA fragment whose putative gene product showed significant homology to valine activation NRPS modules. The sequences of the flanking regions of this DNA fragment revealed a complete module that is predicted to activate valine, as well as a putative carboxyl-terminal thioesterase domain of the NRPS gene. Disruption of the peptide synthetase gene by insertion of a kanamycin cassette through homologous recombination produced cereulide-deficient mutants. The valine-activating module was highly conserved when sequences from nine emetic B. cereus strains isolated from diverse geographical locations were compared. Primers were designed based on the NRPS sequence, and the resulting PCR assay, targeting the ces gene, was tested by using a panel of 143 B. cereus group strains and 40 strains of other bacterial species showing PCR bands specific for only the cereulide-producing B. cereus strains.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.