ArticleApplied and environmental microbiology2005
Simultaneous recovery of extracellular and intracellular DNA suitable for molecular studies from marine sediments.
Article in Applied and environmental microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
66 citing papers in PubMed, 289 citations in OpenAlex.
- Dairy manure reception pits as reservoirs of extracellular DNA-associated antibiotic resistance genes.Microbiology spectrum · 2026Article
- Functional genetic potential of benthic microbial mat communities in Arctic, Antarctic, and sub-Antarctic lakes.FEMS microbiology ecology · 2026Article
- Unravelling Evolutionary and Ecological Insights of Foraminifera by Using Next Generation Sequencing: A Review.Biochemical genetics · 2026Review
- Unveiling Vertebrate Biodiversity in Arid and Semi-Arid Terrestrial Ecosystems Through eDNA Metabarcoding at Savanna Waterholes.Evolutionary applications · 2026Article
- State-Specific Extraction of Environmental DNA: Spike-and-Recovery Controls to Validate and Optimise Extraction Protocols.Environmental microbiology · 2025Article
- Environmental DNA as a tool for hydropower impact assessments: current status, special considerations, and future integration.Biological reviews of the Cambridge Philosophical Society · 2025Review
- Review
- Decoding Past Microbial Communities Shifts Induced by Natural and Anthropogenic Disturbance Events Through Extracellular DNA.Molecular ecology · 2025Article
- Tracking Different States of Spiked Environmental DNA Using Multiplex Digital PCR Assays.Environmental microbiology · 2025Article
- Profiles and natural drivers of antibiotic resistome in multiple environmental media in penguin-colonized area in Antarctica.Fundamental research · 2025Article
- Seasonal Variability of Cultivable Nitrate-Reducing and Denitrifying Bacteria and Functional Gene Copy Number in Fresh Water Lake.Microorganisms · 2024Article
- Metabarcoding reveals unique microbial mat communities and evidence of biogeographic influence in low-oxygen, high-sulfur sinkholes and springs.Ecology and evolution · 2024Article
- DNA and RNA Stability of Marine Microalgae in Cold-Stored Sediments and Its Implications in Metabarcoding Analyses.International journal of molecular sciences · 2024Article
- Polar lake microbiomes have distinct evolutionary histories.Science advances · 2023Article
- An improved method for intracellular DNA (iDNA) recovery from terrestrial environments.MicrobiologyOpen · 2023Article
- CeONanomaterials (Basel, Switzerland) · 2023Article
- Article
- The Origin, Function, Distribution, Quantification, and Research Advances of Extracellular DNA.International journal of molecular sciences · 2022Review
- Enhancement of Acinetobacter baumannii biofilm growth by cephem antibiotics via enrichment of protein and extracellular DNA in the biofilm matrices.Journal of applied microbiology · 2022Article
- Review
6 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The occurrence of high extracellular DNA concentrations in aquatic sediments (concentrations that are 3 to 4 orders of magnitude greater than those in the water column) might play an important role in biogeochemical cycling, as well as in horizontal gene transfer through natural transformation. Since isolation of extracellular DNA from sediments is a difficult and unsolved task, in this study we developed an efficient procedure to recover simultaneously DNA associated with microbial cells and extracellular DNA from the same sediment sample. This procedure is specifically suitable for studying extracellular DNA because it avoids any contamination with DNA released by cell lysis during handling and extraction. Applying this procedure to different sediment types, we obtained extracellular DNA concentrations that were about 10 to 70 times higher than the intracellular DNA concentrations. Using specific targeted prokaryotic primers, we obtained evidence that extracellular DNA recovered from different sediments did not contain amplifiable 16S rRNA genes. By contrast, using DNA extracted from microbial cells as the template, we always amplified 16S rRNA genes. Although 16S rRNA genes were not detected in extracellular DNA, analyses of the sizes of extracellular DNA indicated the presence of high-molecular-weight fragments that might have contained other gene sequences. This protocol allows investigation of extracellular DNA and its possible participation in natural transformation processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.