ArticleThe Journal of physiology2005
Elementary purinergic Ca2+ transients evoked by nerve stimulation in rat urinary bladder smooth muscle.
Article in The Journal of physiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 60 citations in OpenAlex.
- Nerve transfer for restoration of lower motor neuron-lesioned bladder function. Part 1: attenuation of purinergic bladder smooth muscle contractions.American journal of physiology. Regulatory, integrative and comparative physiology · 2021Article
- Purinergic signaling triggers endfoot high-amplitude Ca2+ signals and causes inversion of neurovascular coupling after subarachnoid hemorrhage.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2016Article
- Urothelial purine release during filling of murine and primate bladders.American journal of physiology. Renal physiology · 2016Article
- Purinergic signalling underlies transforming growth factor-β-mediated bladder afferent nerve hyperexcitability.The Journal of physiology · 2016Article
- Constitutively active PKA regulates neuronal acetylcholine release and contractility of guinea pig urinary bladder smooth muscle.American journal of physiology. Renal physiology · 2016Article
- Transient contractions of urinary bladder smooth muscle are drivers of afferent nerve activity during filling.The Journal of general physiology · 2016Article
- Novel mechanism of hydrogen sulfide-induced guinea pig urinary bladder smooth muscle contraction: role of BK channels and cholinergic neurotransmission.American journal of physiology. Cell physiology · 2015Article
- The purinergic neurotransmitter revisited: a single substance or multiple players?Pharmacology & therapeutics · 2014Review
- NS19504: a novel BK channel activator with relaxing effect on bladder smooth muscle spontaneous phasic contractions.The Journal of pharmacology and experimental therapeutics · 2014Article
- Purinergic signalling in the urinary tract in health and disease.Purinergic signalling · 2014Review
- Functional BK channels facilitate the β3-adrenoceptor agonist-mediated relaxation of nerve-evoked contractions in rat urinary bladder smooth muscle isolated strips.European journal of pharmacology · 2013Article
- Signalling to contractile proteins by muscarinic and purinergic pathways in neurally stimulated bladder smooth muscle.The Journal of physiology · 2012Article
- KV2.1 and electrically silent KV channel subunits control excitability and contractility of guinea pig detrusor smooth muscle.American journal of physiology. Cell physiology · 2012Article
- Prostaglandin E2 induces spontaneous rhythmic activity in mouse urinary bladder independently of efferent nerves.British journal of pharmacology · 2012Article
- Calcium signaling in smooth muscle.Cold Spring Harbor perspectives in biology · 2011Review
- Unique properties of muscularis mucosae smooth muscle in guinea pig urinary bladder.American journal of physiology. Regulatory, integrative and comparative physiology · 2011Article
- Aging differentially modifies agonist-evoked mouse detrusor contraction and calcium signals.Age (Dordrecht, Netherlands) · 2011Article
- Ca2+ imaging of activity in ICC-MY during local mucosal reflexes and the colonic migrating motor complex in the murine large intestine.The Journal of physiology · 2010Article
- Nerve-released acetylcholine contracts urinary bladder smooth muscle by inducing action potentials independently of IP3-mediated calcium release.American journal of physiology. Regulatory, integrative and comparative physiology · 2010Article
- Local elementary purinergic-induced Ca2+ transients: from optical mapping of nerve activity to local Ca2+ signaling networks.The Journal of general physiology · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The translation of nerve transmission to Ca2+ signals in urinary bladder smooth muscle (UBSM) is incompletely understood. Thus, we sought to characterize Ca2+ signals in strips of UBSM loaded with the Ca2+-sensitive fluorescent dye, fluo-4, using laser scanning confocal microscopy. Two types of Ca2+ signals occurred spontaneously and could be evoked with field stimulation: large, rapid, global Ca2+ transients termed 'global Ca2+ flashes', and much smaller, localized Ca2+ transients. Global Ca2+ flashes were inhibited by the L-type voltage-dependent Ca2+ channel (VDCC) inhibitor, diltiazem and with P2X receptor blockade. Simultaneous intracellular recordings and Ca2+ measurements indicated that these events are caused by Ca2+ influx through VDCCs during action potentials. Small, local Ca2+ transients occurred spontaneously, and their frequency could be elevated with field stimulation. Atropine, an inhibitor of muscarinic receptors, did not affect these local Ca2+ transients. However, the desensitizing P2X receptor agonist alpha,beta-methylene ATP, and the purinergic antagonist, suramin, effectively inhibited the local Ca2+ transients. The frequency of these 'purinergic Ca2+ transients' was increased about 7-fold by a 10 s stimulus train (1 Hz). The amplitude, duration at one-half amplitude and the spatial spread of the evoked purinergic Ca2+ transients were F/F(o) = 2.4 +/- 0.13, 111.7 +/- 9.3 ms and 14.0 +/- 1.0 microm2, respectively. Tetrodotoxin inhibited evoked purinergic Ca2+ transients, indicating that they were dependent on nerve fibre activation. Purinergic Ca2+ transients were not dependent on VDCC activity. Neither 2-APB, an inhibitor of inositol 1,4,5-triphosphate (Ins(1,4,5)P3) (IP3)-induced Ca2+ release, nor ryanodine inhibited the purinergic Ca2+ transients. We have identified two novel Ca2+ signals in rat UBSM. Large, rapid, global Ca2+ flashes that represent Ca2+ influx through VDCCs during action potentials, and local, purinergic Ca2+ transients that represent Ca2+ entry through P2X receptors. Our results indicate that purinergic Ca2+ transients evoked by release of ATP from nerve varicosities are elementary signals in the process of nerve-smooth muscle communication.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.