ArticleJournal of clinical microbiology2005
Rapid, high-throughput, multiplex, real-time PCR for identification of mutations in the cyp51A gene of Aspergillus fumigatus that confer resistance to itraconazole.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 72 citations in OpenAlex.
- Genotype-Phenotype Relationships in Azole-ResistantJournal of fungi (Basel, Switzerland) · 2026Review
- Therapeutic Switching of Rafoxanide: a New Approach To Fighting Drug-Resistant Bacteria and Fungi.Microbiology spectrum · 2023Article
- Spatial Distribution of a Porphyrin-Based Photosensitizer Reveals Mechanism of Photodynamic Inactivation ofFrontiers in medicine · 2021Article
- Can We Improve Antifungal Susceptibility Testing?Frontiers in cellular and infection microbiology · 2021Review
- High-Frequency Direct Detection of Triazole Resistance inJournal of fungi (Basel, Switzerland) · 2020Article
- Detecting Azole-Antifungal Resistance inJournal of fungi (Basel, Switzerland) · 2020Review
- A Novel Broad Allele-Specific TaqMan Real-Time PCR Method To Detect Triazole-Resistant Strains of Aspergillus fumigatus, Even with a Very Low Percentage of Triazole-Resistant Cells Mixed with Triazole-Susceptible Cells.Journal of clinical microbiology · 2019Article
- A New Age in Molecular Diagnostics for Invasive Fungal Disease: Are We Ready?Frontiers in microbiology · 2019Review
- Antifungal drug resistance: evolution, mechanisms and impact.Current opinion in microbiology · 2018Review
- Drug Sensitivity and Resistance Mechanism in Aspergillus SectionAntimicrobial agents and chemotherapy · 2017Article
- Multiplex qPCR for serodetection and serotyping of hepatitis viruses: A brief review.World journal of gastroenterology · 2016Review
- Blastocystis: Genetic diversity and molecular methods for diagnosis and epidemiology.Tropical parasitology · 2013Article
- Regulatory circuitry governing fungal development, drug resistance, and disease.Microbiology and molecular biology reviews : MMBR · 2011Review
- High-frequency triazole resistance found In nonculturable Aspergillus fumigatus from lungs of patients with chronic fungal disease.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2011Article
- Use of epidemiological cutoff values to examine 9-year trends in susceptibility of Aspergillus species to the triazoles.Journal of clinical microbiology · 2011Article
- Microarray and molecular analyses of the azole resistance mechanism in Candida glabrata oropharyngeal isolates.Antimicrobial agents and chemotherapy · 2010Article
- Use of a high-resolution melt assay to characterize codon 54 of the cyp51A gene of Aspergillus fumigatus on a Rotor-Gene 6000 instrument.Antimicrobial agents and chemotherapy · 2010Article
- Antifungal drug resistance: do molecular methods provide a way forward?Current opinion in infectious diseases · 2009Review
- Wild-type MIC distribution and epidemiological cutoff values for Aspergillus fumigatus and three triazoles as determined by the Clinical and Laboratory Standards Institute broth microdilution methods.Journal of clinical microbiology · 2009Article
- Frequency and evolution of Azole resistance in Aspergillus fumigatus associated with treatment failure.Emerging infectious diseases · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Aspergillus fumigatus is an important cause of life-threatening invasive fungal disease in patients with compromised immune systems. Resistance to itraconazole in A. fumigatus is closely linked to amino acid substitutions in Cyp51A that replace Gly54. In an effort to develop a new class of molecular diagnostic assay that can rapidly assess drug resistance, a multiplexed assay was established. This assay uses molecular beacons corresponding to the wild-type cyp51A gene and seven mutant alleles encoding either Arg54, Lys54, Val54, Trp54, or Glu54. Molecular beacon structure design and real-time PCR conditions were optimized to increase the assay specificity. The multiplex assay was applied to the analysis of chromosomal DNA samples from a collection of 48 A. fumigatus clinical and laboratory-derived isolates, most with reduced susceptibility to itraconazole. The cyp51A allelic identities for codon 54 were established for all of the strains tested, and mutations altering Gly54 in 23 strains were revealed. These mutations included G(54)W (n = 1), G(54)E (n = 12), G(54)K (n = 3), G(54)R (n = 3), and G(54)V (n = 4). Molecular beacon assay results were confirmed by DNA sequencing. Multiplex real-time PCR with molecular beacons is a powerful technique for allele differentiation and analysis of resistance mutations that is dynamic and suitable for rapid high-throughput assessment of drug resistance.
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