Evidence map›Paper›PMID 15634968›Full record

ArticleJournal of clinical microbiology2005

Detection and identification of Mycobacterium tuberculosis in joint biopsy specimens by rpoB PCR cloning and sequencing.

Yeo-Jun Yun, Keun-Hwa Lee, Lin Haihua, Yoon-Jong Ryu, Bum-Joon Kim, Young-Ho Lee, Goo-Hyun Baek, Hee-Joong Kim, Moon-Sang Chung, Myung-Chul Lee and 5 more

Open access · greenAbstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Article
  3. Drug-resistant tubercular uveitis.Journal of clinical microbiology · 2014
    Article
  4. Article
  5. Article
  6. pncA Mutations in the Specimens from Extrapulmonary Tuberculosis.Tuberculosis and respiratory diseases · 2012
    Article
  7. Article
  8. Retroperitoneal abscess: a rare localization of tubercular infection.Gastroenterology research and practice · 2010
    Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors at 5 institutions in 3 countries.

Yeo-Jun YunDepartment of Microbiology and Cancer Research Institute, Seoul National University College of Medicine, Seoul National Research Hospital, Korea.
Keun-Hwa Lee
Lin Haihua
Yoon-Jong Ryu
Bum-Joon Kim
Young-Ho Lee
Goo-Hyun Baek
Hee-Joong Kim
Moon-Sang Chung
Myung-Chul Lee
Sang-Hoon Lee
In-Ho Choi
Tae-Jun Cho
Bong-Soon Chang
Yoon-Hoh Kook
Seoul National University · KRNational University College · PRNew Generation University College · ETSeoul National University Hospital · KRSoonchunhyang University Hospital Seoul · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Osteoarticular tuberculosis (OAT) is an extrapulmonary tuberculosis and accounts for 1 to 3% of all tuberculosis cases. We used an rpoB PCR-plasmid TA cloning-sequencing method to detect and identify tubercle bacilli in surgical specimens from patients suspected of having OAT. By comparing the similarities of the rpoB sequences determined with those in GenBank, Mycobacterium tuberculosis was detected in 23 of 43 samples. Three of the 23 positive samples had mutations at codon 531, which are commonly observed in rifampin-resistant M. tuberculosis strains. Our results suggest that the rpoB PCR-TA cloning-sequencing method developed, which detects M. tuberculosis and which simultaneously determines its rifampin susceptibility, can also be used efficiently for the diagnosis of OAT.

Indexed as

Antitubercular AgentsBiopsyCloning, MolecularDNA-Directed RNA PolymerasesHumansJointsMycobacterium tuberculosisPlasmidsPolymerase Chain ReactionRifampinSequence Analysis, DNATuberculosis, OsteoarticularAntitubercular AgentsDNA-Directed RNA PolymerasesRifampinRNA polymerase beta subunit

Identifiers

PMID15634968
PMCPMC540102
OpenAlexW2105505223

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.