ArticleJournal of clinical microbiology2005
Rapid multiplex assay for serotyping pneumococci with monoclonal and polyclonal antibodies.
Article in Journal of clinical microbiology, 2005. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Integrated and high-throughput method to collect, store, recover, and manage microbial isolates in mini-arrays.Microbiology spectrum · 2025Article
- Modeling of pneumococcal serogroup 10 capsular polysaccharide molecular conformations provides insight into epitopes and observed cross-reactivity.Frontiers in molecular biosciences · 2022Article
- Pneumococci Can Become Virulent by Acquiring a New Capsule From Oral Streptococci.The Journal of infectious diseases · 2020Article
- Development for Clinical Use of a Multiplexed Immunoassay Using Sputum Samples for Streptococcus pneumoniae: a Non-Culture-Based Approach for Serotype-Specific Detection.Journal of clinical microbiology · 2019Article
- Hyperencapsulated mucoid pneumococcal isolates from patients with cystic fibrosis have increased biofilm density and persistence in vivo.Pathogens and disease · 2018Article
- Molecular characterization of pneumococcal surface protein K, a potential pneumococcal vaccine antigen.Virulence · 2017Article
- Spectrum of pneumococcal serotype 11A variants results from incomplete loss of capsule O-acetylation.Journal of clinical microbiology · 2014Article
- Modified opsonization, phagocytosis, and killing assays to measure potentially protective antibodies against pneumococcal surface protein A.Clinical and vaccine immunology : CVI · 2013Article
- Novel pneumococcal serotypes 6C and 6D: anomaly or harbinger.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2012Review
- Biochemical, genetic, and serological characterization of two capsule subtypes among Streptococcus pneumoniae Serotype 20 strains: discovery of a new pneumococcal serotype.The Journal of biological chemistry · 2012Article
- PspA family distribution, unlike capsular serotype, remains unaltered following introduction of the heptavalent pneumococcal conjugate vaccine.Clinical and vaccine immunology : CVI · 2012Article
- Elucidation of structural and antigenic properties of pneumococcal serotype 11A, 11B, 11C, and 11F polysaccharide capsules.Journal of bacteriology · 2011Article
- Streptococcus pneumoniae type determination by multiplex polymerase chain reaction.Journal of Korean medical science · 2011Article
- Rarely occurring 19A-like cps locus from a serotype 19F pneumococcal isolate indicates continued need of serology-based quality control for PCR-based serotype determinations.Journal of clinical microbiology · 2009Article
- Competitive inhibition flow analysis assay for the non-culture-based detection and serotyping of pneumococcal capsular polysaccharide.Clinical and vaccine immunology : CVI · 2009Article
- Cross-sectional study of nasopharyngeal carriage of Streptococcus pneumoniae in human immunodeficiency virus-infected adults in the conjugate vaccine era.Journal of clinical microbiology · 2008Article
- Article
- Microarray for serotyping of Bartonella species.BMC microbiology · 2007Article
- Ten-year surveillance of pneumococcal infections in Temuco, Chile: implications for vaccination strategies.Clinical and vaccine immunology : CVI · 2007Article
- Discovery of a new capsular serotype (6C) within serogroup 6 of Streptococcus pneumoniae.Journal of clinical microbiology · 2007Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
We have developed and characterized a rapid semiautomated pneumococcal serotyping system incorporating a pneumococcal lysate preparation protocol and a multiplex serotyping assay. The lysate preparation incorporates a bile solubility test to confirm pneumococcal identification that also enhances assay specificity. The multiplex serotyping assay consists of 24 assays specific for 36 serotypes: serotypes 1, 2, 3, 4, 5, 6A, 6B, 7A/7F, 8, 9L/9N, 9V, 10A/10B/39/(33C), 11A/11D/11F, 12A/12B/12F, 14, 15B/(15C), 17F, 18C, 19A, 19F, 20, 22A/22F, 23F, and 33A/33F. The multiplex assay requires a flow cytometer, two sets of latex particles coated with pneumococcal polysaccharides, and serotype-specific antibodies. Fourteen newly developed monoclonal antibodies specific for common serotypes and a pool of polyclonal rabbit sera for some of the less-common serotypes are used. The two monoclonal antibodies specific for serotypes 18C and 23F recognize serotype-specific epitopes that have not been previously described. These monoclonal antibodies make the identification of the 14 common serotypes invariant. The specificity of the serotyping assay is fully characterized with pneumococci of all known (i.e., 90) serotypes. The assay is sensitive enough to use bacterial lysates diluted 20 fold. Our serotyping system can identify not only all the serotypes in pneumococcal vaccines but also most (>90%) of clinical isolates. This system should be very useful in serotyping clinical isolates for evaluating pneumococcal vaccine efficacy.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.